Multicomponent polymeric nanoparticles enhancing intracellular drug release in cancer cells.

Abstract

Three kinds of amphiphilic copolymer, that is, poly(ε-caprolactone)-SS-poly(ethylene glycol) (PCL-SS-PEG), poly(ε-caprolactone)-polyethylenimine (PCL-PEI), and poly(ε-caprolactone)-polyethylenimine-folate (PCL-PEI-Fol) were synthesized and self-assembled into surface engineered hybrid nanoparticles (NPs). Morphological studies elucidated the stable, spherical, and uniform sandwich structure of the NPs. PCL-PEI and PCL-SS-PEG segments have introduced pH and reduction responsive characteristics in these NPs, while PCL-PEI-FA copolymers could provide specific targeting capability to cancer cells. The stimuli responsive capabilities of these NPs were carried out. Negative-to-positive charge reversible property, in response to the pH change, was investigated by zeta potential and nuclear magnetic resonance (NMR) measurements. The structure cleavage, due to redox gradient, was studied by dynamic light scattering (DLS) and transmission electron microscopy (TEM). These NPs showed controlled degradation, better drug release, less toxicity, and effective uptake in MCF-7 breast cancer cells. These multifunctional NPs showed promising potential in the treatment of cancer.

• Authors: Arsalan Ahmed, Sen Liu, Yutong Pan, Shanmei Yuan, Jian He, Yong Hu
• Journal: ACS applied materials & interfaces (ACS Appl Mater Interfaces) Vol. 6 Issue 23 Pg. 21316-24 (Dec 10 2014) ISSN: 1944-8252 [Electronic] United States
• PMID: 25333325 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Caproates
• Lactones
• PLC(20)-b-PEO(44)
• Polyethylene Glycols
• caprolactone
• Polyethyleneimine
• Ethylene Oxide

Topics

• Caproates (chemistry)
• Cell Line, Tumor
• Cytoplasm (drug effects)
• Drug Liberation
• Ethylene Oxide (chemistry)
• Humans
• Lactones (chemistry)
• MCF-7 Cells
• Microscopy, Electron, Transmission
• Nanoparticles (administration & dosage, chemistry)
• Neoplasms (drug therapy)
• Polyethylene Glycols (chemistry)
• Polyethyleneimine (chemistry)