Association between Wisteria floribunda agglutinin-positive Mac-2 binding protein and the fibrosis stage of non-alcoholic fatty liver disease.

Abstract

BACKGROUND:

Accurately evaluating liver fibrosis in patients with non-alcoholic fatty liver disease (NAFLD) is important for identifying those who may develop complications. The aims of this study were (1) to measure serum Wisteria floribunda agglutinin-positive Mac-2 binding protein (WFA(+)-M2BP) using the glycan sugar chain-based immunoassay and (2) to compare the results with clinical assessments of fibrosis.

METHODS:

Serum WFA(+)-M2BP values were retrospectively evaluated in 289 patients with NAFLD who had undergone liver biopsy. Histological findings were evaluated by three blinded, experienced liver-specific pathologists.

RESULTS:

For stages 0 (n = 35), 1 (n = 113), 2 (n = 49), 3 (n = 41), and 4 (n = 51) of liver fibrosis, the serum WFA(+)-M2BP cutoff indexes were 0.57, 0.70, 1.02, 1.57, and 2.96, respectively. Multivariate regression analysis showed that serum WFA(+)-M2BP values were associated with the stage of fibrosis (≥stage 2). The areas under the receiver operating characteristic curve (AUROC), sensitivity, and specificity of serum WFA(+)-M2BP were 0.876, 85.9, and 74.6%, respectively, for severe fibrosis (≥stage 3) and were 0.879, 74.6, and 87.0%, respectively, for cirrhosis. When compared with six non-invasive conventional markers, serum WFA(+)-M2BP had the greatest AUROC for diagnosing severe fibrosis and cirrhosis.

CONCLUSIONS:

Serum WFA(+)-M2BP values are useful for assessing the stage of liver fibrosis in patients with NAFLD.

• Authors: Masanori Abe, Teruki Miyake, Atsushi Kuno, Yasuharu Imai, Yoshiyuki Sawai, Keisuke Hino, Yuichi Hara, Shuhei Hige, Michiie Sakamoto, Gotaro Yamada, Masayoshi Kage, Masaaki Korenaga, Yoichi Hiasa, Masashi Mizokami, Hisashi Narimatsu
• Journal: Journal of gastroenterology (J Gastroenterol) Vol. 50 Issue 7 Pg. 776-84 (Jul 2015) ISSN: 1435-5922 [Electronic] Japan
• PMID: 25326152 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Membrane Glycoproteins
• Plant Lectins
• Receptors, N-Acetylglucosamine
• TAA90K protein, human
• wisteria lectin

Topics

• Antigens, Neoplasm (blood)
• Biopsy
• Cross-Sectional Studies
• Disease Progression
• Female
• Humans
• Liver (pathology)
• Liver Cirrhosis (blood, diagnosis, etiology)
• Male
• Membrane Glycoproteins (blood)
• Middle Aged
• Non-alcoholic Fatty Liver Disease (blood, complications, diagnosis)
• Plant Lectins (blood)
• Receptors, N-Acetylglucosamine (blood)
• Retrospective Studies