Abstract |
Human-to-human-transmitted Corynebacterium diphtheriae was historically the main pathogen causing diphtheria and has therefore been studied extensively in the past. More recently, diphtheria caused by toxigenic Corynebacterium ulcerans is an emerging disease in several industrial countries, including the United Kingdom, the United States, France, and Germany. However, toxigenic C. ulcerans has so far been almost neglected in the development of epidemiologic tools. One of the most important tools in modern epidemiology to understand transmission pathways is sequence typing of pathogens. Here, we provide a protocol for multilocus sequence typing (MLST) to type C. ulcerans strains rapidly and relatively cost-effectively. Applying MLST to C. ulcerans for the first time, we show that related sequence types (STs) might be associated with the presence of the diphtheria toxin gene, which encodes diphtheria toxin (DT), the most important diphtheria-causing virulence factor. Interestingly, we found only two very closely related STs in the isolates derived from six dogs. Additionally, our data show that all STs derived from animals which were at least twice present in our analysis were found in humans as well. This finding is congruent with zoonotic transmission of C. ulcerans.
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Authors | Christina König, Dominik M Meinel, Gabriele Margos, Regina Konrad, Andreas Sing |
Journal | Journal of clinical microbiology
(J Clin Microbiol)
Vol. 52
Issue 12
Pg. 4318-24
(Dec 2014)
ISSN: 1098-660X [Electronic] United States |
PMID | 25320226
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014, American Society for Microbiology. All Rights Reserved. |
Chemical References |
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Topics |
- Animals
- Cluster Analysis
- Corynebacterium
(classification, genetics, isolation & purification)
- Corynebacterium Infections
(microbiology, transmission, veterinary)
- Diphtheria Toxin
(genetics)
- Dogs
- Genotype
- Germany
(epidemiology)
- Humans
- Molecular Epidemiology
- Multilocus Sequence Typing
- Prevalence
- Zoonoses
(microbiology, transmission)
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