In vitro anti-inflammatory and wound-healing potential of a Phyllostachys edulis leaf extract--identification of isoorientin as an active compound.

Abstract

Extracts prepared from the leaves of Phyllostachys edulis (bamboo) have received attention in pharmacological research due to their potent antitumor, anti-inflammatory, antimicrobial, and anti-ulcerogenic activities. In this study, anti-inflammatory effects of a bamboo leaf extract on tumor necrosis factor alpha-induced overproduction of interleukin 8, vascular endothelial growth factor, and interleukin 6 in immortalized human keratinocytes were investigated for the first time. In addition, wound-healing effects were evaluated in 3T3-swiss albino mouse fibroblasts. Bamboo leaf extract and isoorientin inhibited the tumor necrosis factor alpha-induced release of interleukin 8 and vascular endothelial growth factor. Furthermore, isoorientin dose-dependently reduced levels of interleukin 6 in tumor necrosis factor alpha-α-treated immortalized human keratinocytes cells. Wound healing was evaluated using a modification of the classical scratch assay. For evaluation of the wound gap, a new computerized method based on time-lapse microscopy was developed. It was shown that bamboo leaf extract (10 µg/mL) improved wound closure by 28 % (12 h) and 54 % (24 h), respectively. In concentrations of 50 µg/mL and above, bamboo leaf extract inhibited cell migration without affecting cell viability. Isoorientin (10 µM) improved wound closure by 29 % (12 h) and 56 % (24 h), respectively. Comparable to bamboo leaf extract, higher concentrations of isoorientin prevented cell migration. It is suggested that bamboo leaf extract as well as isoorientin have a dual activity - in higher doses, they show anti-inflammatory effects, and in lower concentrations, they exert anti-angiogenic activities.

• Authors: Jonas Wedler, Tony Daubitz, Götz Schlotterbeck, Veronika Butterweck
• Journal: Planta medica (Planta Med) Vol. 80 Issue 18 Pg. 1678-84 (Dec 2014) ISSN: 1439-0221 [Electronic] Germany
• PMID: 25317773 (Publication Type: Journal Article)
• Copyright: Georg Thieme Verlag KG Stuttgart · New York.

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• IL6 protein, human
• Interleukin-6
• Interleukin-8
• Plant Extracts
• Tumor Necrosis Factor-alpha
• VEGFA protein, human
• Vascular Endothelial Growth Factor A
• homoorientin
• Luteolin

Topics

• 3T3 Cells (drug effects)
• Animals
• Anti-Inflammatory Agents, Non-Steroidal (chemistry, pharmacology)
• Cell Line (drug effects)
• Dose-Response Relationship, Drug
• Humans
• Interleukin-6 (metabolism)
• Interleukin-8 (metabolism)
• Luteolin (isolation & purification, pharmacology)
• Mice
• Plant Extracts (chemistry, pharmacology)
• Plant Leaves (chemistry)
• Poaceae (chemistry)
• Tumor Necrosis Factor-alpha (pharmacology)
• Vascular Endothelial Growth Factor A (metabolism)
• Wound Healing (drug effects)