Abstract | BACKGROUND: OBJECT: To analyze the correlations of PCA, caspase-14 and cytokines in corneocytes with clinical severity, barrier function and skin inflammation, those were quantitated. METHODS: A total of 73 persons were enrolled: 21 patients with mild AD, 21 with moderate-to-severe AD, 13 with X-linked ichthyosis (XLI) as a negative control for filaggrin gene (FLG) mutation, and 18 healthy controls. Skin barrier functions such as basal transepidermal water loss (TEWL), stratum corneum (SC) hydration and skin surface pH were measured. To collect corneocytes, stripping with D-squame discs was done on lesional and non-lesional skin. And then PCA was isolated from D-squame discs and quantitated by LC-MS/MS. Cytokine assays were performed. RESULTS: The quantity of PCA and caspase-14 was decreased in inflammatory lesions compared to non-lesion in AD patients. And the amounts of PCA and caspase-14 in the lesion of AD patients correlated with clinical severity as determined by eczema area and severity index score and the skin barrier functions. Also, the expressions of TNF-α and IL-13 inversely correlated with PCA quantity. CONCLUSION: The quantity of PCA or caspase-14 in the corneocytes of the lesional skin of AD patients reflects the clinical severity, skin barrier function and the degree of lesional inflammation.
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Authors | Minyoung Jung, Jaewoong Choi, Seon-Ah Lee, Hyunjung Kim, Joonsung Hwang, Eung Ho Choi |
Journal | Journal of dermatological science
(J Dermatol Sci)
Vol. 76
Issue 3
Pg. 231-9
(Dec 2014)
ISSN: 1873-569X [Electronic] Netherlands |
PMID | 25315296
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- FLG protein, human
- Filaggrin Proteins
- IL13 protein, human
- Interleukin-13
- Interleukin-1beta
- Intermediate Filament Proteins
- Tumor Necrosis Factor-alpha
- CASP14 protein, human
- Caspases
- Pyrrolidonecarboxylic Acid
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Topics |
- Adolescent
- Adult
- Case-Control Studies
- Caspases
(metabolism)
- Child
- Child, Preschool
- Dermatitis, Atopic
(genetics, metabolism, pathology)
- Epidermis
(metabolism, pathology)
- Female
- Filaggrin Proteins
- Humans
- Ichthyosis, X-Linked
(genetics, metabolism, pathology)
- Interleukin-13
(metabolism)
- Interleukin-1beta
(metabolism)
- Intermediate Filament Proteins
(genetics)
- Male
- Mutation
- Pyrrolidonecarboxylic Acid
(metabolism)
- Tumor Necrosis Factor-alpha
(metabolism)
- Young Adult
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