Abstract |
This study aimed to identify novel ACE inhibitory peptides from the muscle of cuttlefish. Proteins were hydrolyzed and the hydrolysates were then subjected to various types of chromatography to isolate the active peptides. Nine ACE inhibitory peptides were isolated and their molecular masses and amino acid sequences were determined using ESI-MS and ESI-MS/MS, respectively. The structures of the most potent peptides were identified as Val-Glu- Leu-Tyr-Pro, Ala-Phe-Val- Gly-Tyr- Val-Leu-Pro and Glu-Lys-Ser-Tyr-Glu- Leu-Pro. The first peptide displayed the highest ACE inhibitory activity with an IC50 of 5.22μM. Lineweaver-Burk plots suggest that Val-Glu- Leu-Tyr-Pro acts as a non-competitive inhibitor against ACE. Furthermore, antihypertensive effects in spontaneously hypertensive rats (SHR) also revealed that oral administration of Val-Glu- Leu-Tyr-Pro can decrease systolic blood pressure significantly (p<0.01). These results suggest that the Val-Glu- Leu-Tyr-Pro would be a beneficial ingredient for nutraceuticals and pharmaceuticals acting against hypertension and its related diseases.
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Authors | Rafik Balti, Ali Bougatef, Assaâd Sila, Didier Guillochon, Pascal Dhulster, Naima Nedjar-Arroume |
Journal | Food chemistry
(Food Chem)
Vol. 170
Pg. 519-25
(Mar 01 2015)
ISSN: 1873-7072 [Electronic] England |
PMID | 25306378
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Crown Copyright © 2013. Published by Elsevier Ltd. All rights reserved. |
Chemical References |
- Angiotensin-Converting Enzyme Inhibitors
- Antihypertensive Agents
- Peptides
- Protein Hydrolysates
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Topics |
- Amino Acid Sequence
- Angiotensin-Converting Enzyme Inhibitors
(analysis, isolation & purification, pharmacology)
- Animals
- Antihypertensive Agents
(chemistry, isolation & purification, pharmacology)
- Blood Pressure
(drug effects)
- Chromatography, Gel
- Decapodiformes
(metabolism)
- Hypertension
(pathology)
- Male
- Molecular Weight
- Muscles
(chemistry, enzymology)
- Peptides
(analysis, isolation & purification, pharmacology)
- Protein Hydrolysates
(chemistry)
- Protein Stability
- Rats
- Rats, Inbred SHR
- Spectrometry, Mass, Electrospray Ionization
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