[Antitumor enzyme L-lyzine-alpha-oxidase from Trichoderma harzianum Rifai F-180 and investigation of its action on L-lysine oxidation by capillar electrophoresis].

Abstract

Trichoderma harzianum Rifai F-180, an organism producing the antitumor enzyme L-lysine-alpha-oxidase was cultivated and the enzyme was isolated and purified under the manufacturing conditions. The effect of L-lysine-alpha-oxidase on oxidation of L-lysine was investigated for the first time by capillary electrophoresis and the procedure conditions were developed. The reaction of L-lysine oxidative deamination is described and location of the reaction components picks on the elecrophoregrams was identified. The average rate of the catalytic reaction of L-lysine oxidation equal to 0.46 RU/min (7.7 x 10(-3) RU/sec) was determined. The use of the antitumor enzyme L-lysine-alpha-oxidase is recommended as a drug for the treatment of superficial tumors and tissue relative oxygen excess.

• Authors: I P Smirnova, V M Shkinev, A V Rudnev, O M Kuznetsova, E V Karimova, V S Orlova
• Journal: Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic] (Antibiot Khimioter) Vol. 59 Issue 3-4 Pg. 12-5 ( 2014) ISSN: 0235-2990 [Print] Russia (Federation)
• PMID: 25300116 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antineoplastic Agents
• Fungal Proteins
• Amino Acid Oxidoreductases
• L-lysine oxidase
• Lysine

Topics

• Amino Acid Oxidoreductases (chemistry, isolation & purification)
• Antineoplastic Agents (chemistry, isolation & purification)
• Electrophoresis, Capillary
• Electrophoresis, Polyacrylamide Gel
• Enzyme Assays
• Fungal Proteins (chemistry, isolation & purification)
• Kinetics
• Lysine (chemistry)
• Oxidation-Reduction
• Trichoderma (chemistry, enzymology)