[Studies on the serological activity of ND-O-BSA and its application in serodiagnosis of leprosy].

Abstract

In this article, optimum conditions of ND-O-BSA-ELISA were determined and comparisons made of the serologic activity of 3 different types of antigens (WML, PGI, ND-O-BSA) in leprosy (151) and tuberculosis (20) patients and normal controls (42). The results indicated that: 1) optimum conditions for ND-O-BSA-ELISA were ND-O-BSA and OPD concentration of 0.1 and 400 micrograms/ml respectively with HRP-IgM and test sera dilutions of 1:1000 and 1:200; and 2) serological activity showed highly significant positive correlations between 3 types of antigens (WML vs PGI: r = 0.88, P less than 0.0005; PGI vs ND-O-BSA: r = 0.86, P less than 0.0005), all of which were highly sensitive and specific for leprosy serodiagnosis with positive rates of 100% (PGI) and 97.4% (WML, ND-O-BSA) in leprosy patients. None were positive in normal controls, but crossreactivity of different degree were present in tuberculosis patients. The authors suggest that PGI-ELISA and ND-O-BSA-ELISA be used in combination in the evaluation of clinical therapeutic efficacy and determination of disease stages, as well as in epidemiologic survey of subclinical leprosy infection in "high-risk" areas.

• Authors: Q X Wu
• Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (Zhongguo Yi Xue Ke Xue Yuan Xue Bao) Vol. 11 Issue 3 Pg. 220-4 (Jun 1989) ISSN: 1000-503X [Print] China
• PMID: 2529991 (Publication Type: Journal Article)

Chemical References

• Antigens, Bacterial
• Disaccharides
• Glycolipids
• O-(3,6-di-O-methylglucopyranosyl)-(1-4)-O-(2,3-di-O-methylrhamnopyranosyl)-(1-9)-oxynonanoyl-bovine serum albumin
• phenolic glycolipid I, Mycobacterium leprae
• Serum Albumin, Bovine

Topics

• Antigens, Bacterial (analysis)
• Disaccharides
• Enzyme-Linked Immunosorbent Assay
• Glycolipids (analysis)
• Humans
• Leprosy (diagnosis)
• Mycobacterium leprae
• Serum Albumin, Bovine (analysis)
• Tuberculosis (diagnosis)