Distinct spatial relationship of the interleukin-9 receptor with interleukin-2 receptor and major histocompatibility complex glycoproteins in human T lymphoma cells.

Abstract

The interleukin-9 receptor (IL-9R) consists of an α subunit and a γ(c) chain that are shared with other cytokine receptors, including interleukin-2 receptor (IL-2R), an important regulator of T cells. We previously showed that IL-2R is expressed in common clusters with major histocompatibility complex (MHC) glycoproteins in lipid rafts of human T lymphoma cells, which raised the question about what the relationship between clusters of IL-2R/MHC and IL-9R is. Confocal microscopy colocalization and fluorescence resonance energy transfer experiments capable of detecting membrane protein organization at different size scales revealed nonrandom association of IL-9R with IL-2R/MHC clusters at the surface of human T lymphoma cells. Accommodation of IL-9Rα in membrane areas segregated from the IL-2R/MHC domains was also detected. The bipartite nature of IL-9R distribution was mirrored by signal transducer and activator of transcription (STAT) activation results. Our data indicate that co-compartmentalization with MHC glycoproteins is a general property of γ(c) receptors. Distribution of receptor chains between different membrane domains may regulate their function.

• Authors: Enikő Nizsalóczki, István Csomós, Péter Nagy, Zsolt Fazekas, Carolyn K Goldman, Thomas A Waldmann, Sándor Damjanovich, György Vámosi, László Mátyus, Andrea Bodnár
• Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (Chemphyschem) Vol. 15 Issue 18 Pg. 3969-78 (Dec 15 2014) ISSN: 1439-7641 [Electronic] Germany
• PMID: 25297818 (Publication Type: Journal Article, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Glycoproteins
• HLA Antigens
• Receptors, Interleukin-2
• Receptors, Interleukin-9

Topics

• Cell Line, Tumor
• Fluorescence Resonance Energy Transfer
• Glycoproteins (analysis)
• HLA Antigens (analysis)
• Humans
• Lymphoma, T-Cell (pathology)
• Major Histocompatibility Complex
• Microscopy, Confocal
• Receptors, Interleukin-2 (analysis)
• Receptors, Interleukin-9 (analysis)
• T-Lymphocytes (chemistry, pathology)