CCNA1 promoter methylation: a potential marker for grading Papanicolaou smear cervical squamous intraepithelial lesions.

Abstract

BACKGROUND:

From our previous study, we established that cyclin A1 (CCNA1) promoter methylation is strongly correlated with multistep progression of HPV-associated cervical cancer, suggesting potential use as a diagnostic maker of disease.

OBJECTIVES:

The purpose of the present study was to assess the prevalence of CCNA1 promoter methylation in residual cervical cells isolated from liquid-based cytology that underwent hrHPV DNA screening for cervical cancer, and then to evaluate this marker for diagnostic accuracy using parameters like sensitivity, specificity, predictive values and likelihood ratio.

METHODS:

In this retrospective study, histopathology was used as the gold standard method with specimens separated into the following groups: negative (n=31), low- grade squamous intraepithelial lesions (LSIL, n=34) and high-grade squamous intraepithelial lesions or worse (HSIL+, n=32). The hrHPV was detected by Hybrid Capture 2 (HC2) and CCNA1 promoter methylation was examined by CCNA1 duplex methylation specific PCR.

RESULTS:

The results showed the frequencies of CCNA1 promoter methylation were 0%, 5.88% and 83.33%, while the percentages of hrHPV were 66.67%, 82.35% and 100% in the negative, LSIL and HSIL+ groups, respectively. Although hrHPV infection showed high frequency in all three groups, it could not differentiate between the different groups and grades of precancerous lesions. In contrast, CCNA1 promoter methylation clearly distinguished between negative/LSIL and HSIL+, with high levels of all statistic parameters.

CONCLUSION:

CCNA1 promoter methylation is a potential marker for distinguishing between histologic negative/LSIL and HSIL+using cervical cytology samples.

• Authors: Suthipong Chujan, Nakarin Kitkumthorn, Sumalee Siriangkul, Apiwat Mutirangura
• Journal: Asian Pacific journal of cancer prevention : APJCP (Asian Pac J Cancer Prev) Vol. 15 Issue 18 Pg. 7971-5 ( 2014) ISSN: 2476-762X [Electronic] Thailand
• PMID: 25292097 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CCNA1 protein, human
• Cyclin A1

Topics

• Adult
• Cyclin A1 (genetics)
• DNA Methylation
• Female
• Follow-Up Studies
• Humans
• Middle Aged
• Neoplasm Grading
• Neoplasms, Squamous Cell (genetics, pathology, virology)
• Papanicolaou Test (methods)
• Papillomaviridae (genetics)
• Papillomavirus Infections (complications, genetics, pathology)
• Polymerase Chain Reaction
• Prognosis
• Promoter Regions, Genetic (genetics)
• Retrospective Studies
• Uterine Cervical Neoplasms (genetics, pathology, virology)
• Uterine Cervical Dysplasia (genetics, pathology, virology)