Abstract | BACKGROUND: METHODS: Forty Wistar Albino rats were divided into five groups: Group I, sham operation; Group II, ischemia alone, Group III, ischemia-reperfusion; Group IV, ischemia-reperfusion+50 mg/kg EP; and Group V, ischemia-reperfusion+100 mg/kg EP. Through laparotomy, 360° torsion was performed and maintained for 3 h, and detorsion was maintained for a further 3 h. Intraperitoneal EP was given 30 min before the surgical procedure. Ovarian tissues and blood samples were obtained after surgery. Malondialdehyde (MDA) and asymmetric dimethyl arginine (ADMA) levels were measured in both plasma and tissue samples. Tissue sections were evaluated histopatologically. Analysis of variance was used for statistical analyses. RESULTS: In both serum and tissue samples, ADMA and MDA levels were found to be significantly lower in the EP groups compared with the ischemia alone and ischemia-reperfusion groups (p=0.0001). However, no significant difference was found between groups treated with 50 mg/kg or 100 mg/kg EP. On histopathological evaluation, the total tissue injury score was found to be lower in rats treated with EP. No significant difference was detected between groups treated with 50 mg/kg or 100 mg/kg EP. CONCLUSION:
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Authors | Emel Kiyak Caglayan, Kasim Caglayan, Ayse Yesim Göcmen, Hamza Cinar, Levent Seckin, Selda Seckin, Bülent Güngör, Muhammet Fevzi Polat |
Journal | European journal of obstetrics, gynecology, and reproductive biology
(Eur J Obstet Gynecol Reprod Biol)
Vol. 182
Pg. 154-9
(Nov 2014)
ISSN: 1872-7654 [Electronic] Ireland |
PMID | 25291349
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Antioxidants
- Pyruvates
- dimethylarginine
- ethyl pyruvate
- Malondialdehyde
- Arginine
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Topics |
- Animals
- Antioxidants
(administration & dosage, therapeutic use)
- Arginine
(analogs & derivatives, metabolism)
- Disease Models, Animal
- Female
- Malondialdehyde
(metabolism)
- Ovary
(blood supply, metabolism)
- Pyruvates
(administration & dosage, therapeutic use)
- Rats
- Rats, Wistar
- Reperfusion Injury
(etiology, metabolism, pathology, prevention & control)
- Torsion Abnormality
(complications)
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