Abstract |
The increased risk and persistence of infections in diabetic condition is probably associated with defects in the cellular immune responses. We have previously shown a decrease in the production of interferon (IFN)-α by dendritic cells (DCs) in diabetic subjects. The basal level of IFN-α in splenic plasmacytoid DCs (pDCs) is also lower in non-obese diabetic (NOD) mice compared to prediabetic mice. The objective of this study was to analyse the ability of diabetic mice to mobilize innate and CD8(+) T cell-mediated immune response to influenza A virus (IAV) with the live influenza A/Puerto Rico/8/1934 H1N1 (PR8) strain or with its immunodominant CD8(+) T cell epitopes. We found that following immunization with IAV, the level of IFN-α in diabetic mice was increased to the level in prediabetic mice. Immunization of NOD mice with the immunodominant IAV PR8 peptide induced clonal expansion of IFN-γ-producing CD8(+) T cells similar to the response observed in prediabetic mice. Thus, diabetic and prediabetic NOD mice have a similar capacity for IFN-α and IFN-γ production by pDCs and CD8(+) T cells, respectively. Therefore, the DC-related immune defect in diabetic NOD mice does not impair their capacity to develop an effective immune response to IAV. Our results suggest that reduced IFN-α production by diabetic human and mouse DCs is not an impediment to an effective immunity to IAV in type 1 diabetic subjects vaccinated with live attenuated influenza vaccine.
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Authors | D Kreuzer, E Nikoopour, B C Y Au, O Krougly, E Lee-Chan, K L Summers, S M M Haeryfar, B Singh |
Journal | Clinical and experimental immunology
(Clin Exp Immunol)
Vol. 179
Issue 2
Pg. 245-55
(Feb 2015)
ISSN: 1365-2249 [Electronic] England |
PMID | 25286929
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 British Society for Immunology. |
Chemical References |
- Interferon-alpha
- Peptides
- Viral Proteins
- Interferon-gamma
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Topics |
- Animals
- CD8-Positive T-Lymphocytes
(immunology, pathology)
- Dendritic Cells
(immunology, pathology)
- Diabetes Mellitus, Type 1
(immunology, pathology)
- Humans
- Immunity, Cellular
- Immunization
- Influenza A Virus, H1N1 Subtype
(immunology)
- Interferon-alpha
(immunology)
- Interferon-gamma
(pharmacology)
- Mice
- Mice, Inbred NOD
- Orthomyxoviridae Infections
(immunology)
- Peptides
(immunology, pharmacology)
- Viral Proteins
(immunology, pharmacology)
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