Chloride intracellular channel 1 regulates prostate cancer cell proliferation and migration through the MAPK/ERK pathway.

Abstract

UNLABELLED:

Abstract Aims: To investigate the effect of chloride intracellular channel 1 (CLIC1) on the proliferation, migration, and apoptosis of prostate cancer cell lines PC-3 and DU145 and the possible molecular mechanisms.

MATERIALS AND METHODS:

Using the technique of RNA interference, the expression of CLIC1 was downregulated in the PC-3 and DU145 cell lines. MTT assay, Transwell chamber, and flow cytometry were used to determine the effect of CLIC1 on the proliferation, migration, and apoptosis ability of PC-3 and DU145 cells. The levels of phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK1/2), ERK1/2, matrix metalloproteinase (MMP)-2, and MMP-9 were examined by western blotting.

RESULTS:

The results showed that the knockdown of CLIC1 exerts inhibitory effects on the proliferation and migration of PC-3 and DU145 cells. At the same time, the authors found that the knockdown of CLIC1 has no effect on the apoptosis in PC-3 and DU145 cells. Meanwhile, the levels of p-ERK1/2, MMP-2, and MMP-9 were decreased in the CLIC1 small interfering RNA (siRNA) group compared with the control and vector groups.

CONCLUSION:

These results indicate that CLIC1 could regulate prostate cancer cell proliferation and migration by regulating the mitogen-activated protein kinase (MAPK)/ERK pathway and offers a candidate molecular target for prostate cancer prevention and therapy.

• Authors: Yudong Tian, Yanbin Guan, Yongyan Jia, Qingjun Meng, Jinjian Yang
• Journal: Cancer biotherapy & radiopharmaceuticals (Cancer Biother Radiopharm) Vol. 29 Issue 8 Pg. 339-44 (Oct 2014) ISSN: 1557-8852 [Electronic] United States
• PMID: 25279971 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CLIC1 protein, human
• Chloride Channels
• Mitogen-Activated Protein Kinase 1
• Mitogen-Activated Protein Kinase 3
• MMP2 protein, human
• Matrix Metalloproteinase 2
• MMP9 protein, human
• Matrix Metalloproteinase 9

Topics

• Apoptosis (physiology)
• Cell Growth Processes (physiology)
• Cell Line, Tumor
• Cell Movement (physiology)
• Chloride Channels (genetics, metabolism)
• Humans
• MAP Kinase Signaling System (physiology)
• Male
• Matrix Metalloproteinase 2 (metabolism)
• Matrix Metalloproteinase 9 (metabolism)
• Mitogen-Activated Protein Kinase 1 (metabolism)
• Mitogen-Activated Protein Kinase 3 (metabolism)
• Prostatic Neoplasms (enzymology, genetics, metabolism, pathology)
• RNA Interference
• Transfection