Abstract | INTRODUCTION: Determining the genetic similarities among Trypanosoma cruzi populations isolated from different hosts and vectors is very important to clarify the epidemiology of Chagas disease. METHODS: An epidemiological study was conducted in a Brazilian endemic area for Chagas disease, including 76 chronic chagasic individuals (96.1% with an indeterminate form; 46.1% with positive hemoculture). RESULTS: T. cruzi I (TcI) was isolated from one child and TcII was found in the remaining (97.1%) subjects. Low-stringency single-specific-primer-polymerase chain reaction (LSSP-PCR) showed high heterogeneity among TcII populations (46% of shared bands); however, high similarities (80-100%) among pairs of mothers/children, siblings, or cousins were detected. CONCLUSIONS: LSSP-PCR showed potential for identifying similar parasite populations among individuals with close kinship in epidemiological studies of Chagas disease.
|
Authors | Sandra Maria Alkmim-Oliveira, Henrique Borges Kappel, Cristiane Pontes Andrade, Aluízio Prata, Luis Eduardo Ramirez, Dalmo Correia, Eliane Lages-Silva |
Journal | Revista da Sociedade Brasileira de Medicina Tropical
(Rev Soc Bras Med Trop)
2014 Sep-Oct
Vol. 47
Issue 5
Pg. 653-6
ISSN: 1678-9849 [Electronic] Brazil |
PMID | 25271788
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- DNA, Kinetoplast
- DNA, Protozoan
|
Topics |
- Adolescent
- Adult
- Brazil
(epidemiology)
- Chagas Disease
(epidemiology, parasitology)
- Child
- Child, Preschool
- DNA, Kinetoplast
(genetics)
- DNA, Protozoan
- Female
- Genetic Variation
- Genotype
- Humans
- Male
- Middle Aged
- Polymerase Chain Reaction
- Trypanosoma cruzi
(genetics, isolation & purification)
- Young Adult
|