miR‑494 is an independent prognostic factor and promotes cell migration and invasion in colorectal cancer by directly targeting PTEN.

Abstract

Accumulating evidence has shown that micro-RNAs (miRNAs) are involved in multiple processes in cancer development and progression. Upregulation of miRNA-494 (miR-494) has been identified as an oncogenic miRNA and is associated with poor prognosis in several types of human cancer. However, the specific function of miR-494 in colorectal cancer remains unclear. In this study we found that the expression of miR-494 in colorectal cancer tissues and cell lines was much higher than in normal control tissues and cells, respectively. In addition, upregulation of miR-494 more frequently occurred in tissue specimens with adverse clinical stage and the presence of distant metastasis. Moreover, multivariate survival analyses demonstrated that overexpression of miR-494 is an independent prognostic factor for both progression-free and overall survival. In addition miR-494 promoted invasion and migration in colorectal cancer cells, and miR-494 directly inhibited the phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression by targeting its 3'-untranslated region (3'-UTR). Moreover, PTEN is down regulated and inversely correlated with miR-494 expression in tissues. Thus, for the first time, we provided convincing evidence that upregulation of miR-494 was associated with tumor aggressiveness and tumor metastasis and promoted cell migration and invasion by targeting PTEN gene in colorectal cancer, and miR-494 is an independent prognostic marker for colorectal cancer patients.

• Authors: Hai-Bing Sun, Xi Chen, Hong Ji, Tao Wu, Hong-Wei Lu, Yan Zhang, Hua Li, Yi-Ming Li
• Journal: International journal of oncology (Int J Oncol) Vol. 45 Issue 6 Pg. 2486-94 (Dec 2014) ISSN: 1791-2423 [Electronic] Greece
• PMID: 25270723 (Publication Type: Journal Article)

Chemical References

• 3' Untranslated Regions
• MIRN494 microRNA, human
• MicroRNAs
• PTEN Phosphohydrolase
• PTEN protein, human

Topics

• 3' Untranslated Regions
• Adult
• Aged
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• Colorectal Neoplasms (genetics, pathology)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Male
• MicroRNAs (biosynthesis, genetics)
• Middle Aged
• Neoplasm Invasiveness (genetics)
• PTEN Phosphohydrolase (biosynthesis, genetics)
• RNA Interference