Abstract | PURPOSE: METHODS: Ophthalmologic data and sera were collected from all patients, who underwent visual field (VF) examination, optical coherence tomography (OCT), and multifocal electroretinogram (mfERG) recording. Serum was prepared from each patient's blood and analyzed for antiretinal antibody activity using immunohistochemistry and Western blot analysis on mouse and human retinas. We also searched for antigen proteins using mass spectrometry. RESULTS: Symptoms were blurred vision in three patients and VF defects in two; all had enlargement of the Mariotte blind spot by VF testing. OCT findings in areas corresponding to the scotoma revealed disruptions of junction borders between inner and outer segment lines. mfERGs amplitudes were reduced in each corresponding scotoma area. Immunohistochemical serum staining revealed the target antigen was present in all photoreceptors of the mouse sensory retina. Western blot analysis using patient serum samples revealed some possible candidate antigens. Mass spectrometry could not determine the causative antigen; however, a list of candidates was discovered. CONCLUSION:
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Authors | Mizuki Tagami, Wataru Matsumiya, Hisanori Imai, Sentaro Kusuhara, Shigeru Honda, Atsushi Azumi |
Journal | Japanese journal of ophthalmology
(Jpn J Ophthalmol)
Vol. 58
Issue 6
Pg. 462-72
(Nov 2014)
ISSN: 1613-2246 [Electronic] Japan |
PMID | 25266678
(Publication Type: Case Reports, Journal Article)
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Chemical References |
- Autoantibodies
- Autoantigens
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Topics |
- Adult
- Aged
- Animals
- Autoantibodies
(blood)
- Autoantigens
(immunology)
- Autoimmune Diseases
(diagnosis, immunology, physiopathology)
- Blotting, Western
- Child
- Electrophoresis, Polyacrylamide Gel
- Electroretinography
- Female
- Humans
- Male
- Mice
- Mice, Inbred C57BL
- Scotoma
(diagnosis, immunology, physiopathology)
- Tandem Mass Spectrometry
- Tomography, Optical Coherence
- Visual Acuity
(physiology)
- Visual Fields
(physiology)
- White Dot Syndromes
- Young Adult
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