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Emodin azide methyl anthraquinone derivative induced G0/ G1 arrest in HER2/neu-overexpressing MDA-MB-453 breast cancer cells.

AbstractPURPOSE:
Our previous data have shown that emodin azide methyl anthraquinone derivative (AMAD) triggered mitochondrial- dependent cell apoptosis involving caspase-8-mediated Bid cleavage, and induced proteasomal degradation of HER2/neu by blocking Her2/neu binding to Hsp90. In the present study, we futher investigated the effect of this compound on the cell cycle and related molecular mechanisms in HER2/neu-overexpressing MDA-MB-453 breast cancer cells.
METHODS:
The cell cycle distribution was tested by flow cytometry. The expression of cell cycle-related proteins was determined by Western blot analysis; DNA agarose gel electrophoresis was used to examine the apoptosis of MDAMB- 453 cells induced by emodin AMAD.
RESULTS:
After MDA-MB-453 cells were treated with different concentrations of emodin AMAD for 24 hrs, cells were arrested in G0/G1 phase, and the expression of G0/G1 related proteins c/Myc, Cyclin D1, CDK4 and p-Rb changed. DNA fragmentation appeared on the agarose gel in a concentration- dependent manner.
CONCLUSION:
Emodin AMAD induced G0/G1 arrest in Her2/ neu-overexpressing MDA-MB-453 cancer cells. This G0/G1 arrest was associated with decreasing protein expression of c-Myc, Cyclin D1, CDK4, and p-Rb.
AuthorsYan-yan Yan, Li-wu Fu, Wei Zhang, Hong-shan Ma, Cun-gen Ma, Yong-ju Liang, Bin-yu Liu, Jie-zhong Yu, Qiu-zhen Wu, Yi-min Dong
JournalJournal of B.U.ON. : official journal of the Balkan Union of Oncology (J BUON) 2014 Jul-Sep Vol. 19 Issue 3 Pg. 650-5 ISSN: 1107-0625 [Print] Cyprus
PMID25261647 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • 6-azidomethyl-1-hydroxy-3,8-dimethoxy-9,10-anthraquinone
  • Anthraquinones
  • Antineoplastic Agents
  • Azides
  • CCND1 protein, human
  • Cyclin D1
  • ERBB2 protein, human
  • Receptor, ErbB-2
  • CDK4 protein, human
  • Cyclin-Dependent Kinase 4
  • Emodin
Topics
  • Anthraquinones (pharmacology)
  • Antineoplastic Agents (pharmacology)
  • Apoptosis (drug effects)
  • Azides (pharmacology)
  • Cell Cycle Checkpoints (drug effects)
  • Cell Line, Tumor
  • Cyclin D1 (analysis)
  • Cyclin-Dependent Kinase 4 (analysis)
  • Emodin (analogs & derivatives, pharmacology)
  • Female
  • G1 Phase (drug effects)
  • Humans
  • Receptor, ErbB-2 (analysis)
  • Resting Phase, Cell Cycle (drug effects)

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