Abstract | OBJECTIVES: MATERIAL AND METHODS: This study analyses the influence of the isoprenoids eugenol, farnesol, R-limonene, menthol and squalene on different functions of zoledronate-treated human umbilicord vein endothelial cells (HUVEC), fibroblasts and osteogenic cells. In addition to the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl 2H-tetrazolium bromide (MTT) vitality test, the migration capacity was analysed by scratch wound assay and the morphological architecture of the treated cells by phallacidin staining. RESULTS: In contrast to GGOH, none of the other tested isoprenoids were able to prevent cells from having negative zoledronate effects. CONCLUSIONS: Despite structural analogy to GGOH, the investigated isoprenoids are not able to prevent the N-BP effect. The negative impact of zoledronate on fibroblasts, HUVEC and osteogenic cells is due to inhibition of protein geranylgeranylation since the substitution of squalene and farnesyl did not have any effect on viability and wound healing capacity whereas GGOH did reduce the negative impact. CLINICAL RELEVANCE:
|
Authors | Nadine Hagelauer, Thomas Ziebart, Andreas M Pabst, Christian Walter |
Journal | Clinical oral investigations
(Clin Oral Investig)
Vol. 19
Issue 5
Pg. 1079-91
(Jun 2015)
ISSN: 1436-3771 [Electronic] Germany |
PMID | 25261400
(Publication Type: Journal Article)
|
Chemical References |
- Bone Density Conservation Agents
- Cyclohexenes
- Diphosphonates
- Diterpenes
- Imidazoles
- Terpenes
- Menthol
- Eugenol
- Farnesol
- Zoledronic Acid
- Squalene
- Limonene
- geranylgeraniol
|
Topics |
- Bisphosphonate-Associated Osteonecrosis of the Jaw
(prevention & control)
- Bone Density Conservation Agents
(toxicity)
- Cell Movement
(drug effects)
- Cell Survival
(drug effects)
- Cells, Cultured
- Cyclohexenes
(pharmacology)
- Diphosphonates
(toxicity)
- Diterpenes
(pharmacology)
- Endothelial Cells
(drug effects)
- Eugenol
(pharmacology)
- Farnesol
(pharmacology)
- Fibroblasts
(drug effects)
- Humans
- Imidazoles
(toxicity)
- Limonene
- Menthol
(pharmacology)
- Osteogenesis
(drug effects)
- Protein Prenylation
- Recurrence
- Squalene
(pharmacology)
- Terpenes
(pharmacology)
- Umbilical Veins
(cytology)
- Zoledronic Acid
|