Abstract |
Usnic acid, a lichen metabolite, has been proposed as a potential topical treatment for microbial skin lesions, burn wounds as well as a sunscreen. An isocratic HPLC method was validated according to FDA's Guidance for Industry: Bioanalytical Method Validation to determine skin penetration and permeation of usnic acid. The penetration and permeation of usnic acid was evaluated using Franz cells and porcine skin. The method was valid according to selectivity, linearity, precision, accuracy and stability. Usnic acid was quantified in the skin surface (6.13 µg cm(2)), stratum corneum (34.4 µg cm(2)), viable epidermis (5.6 µg cm(2)), dermis (28.2 µg cm(2)) and receptor compartment (3.2 µg cm(2)). These results help us to understand the penetration profile of usnic acid and plan topical therapeutic approaches as well as new topical delivery systems to modulate this penetration profile.
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Authors | Mairim Russo Serafini, Cassia Britto Detoni, Sílvia Stanisçuaski Guterres, Gabriel Francisco da Silva, Adriano Antunes de Souza Araújo |
Journal | Journal of chromatographic science
(J Chromatogr Sci)
2015 May-Jun
Vol. 53
Issue 5
Pg. 757-60
ISSN: 1945-239X [Electronic] United States |
PMID | 25260845
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Validation Study)
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Copyright | © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please email: [email protected]. |
Chemical References |
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Topics |
- Administration, Topical
- Animals
- Benzofurans
(administration & dosage, pharmacokinetics)
- Chromatography, High Pressure Liquid
(methods)
- Drug Delivery Systems
- Permeability
- Skin
(drug effects, metabolism)
- Swine
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