A new technique of in vivo synchrotron radiation coronary microangiography in the rat.

Abstract

BACKGROUND:

Previously, in our laboratory, synchrotron radiation coronary microangiography (SRCA) using Langendorff-perfused rat hearts could visualize a coronary artery of 50 µm in diameter. However, in vivo rat SRCA poses the problem of compromised temporal resolution due to the rapid heart rate of rats.

PURPOSE:

To establish a simple method of in vivo rat SRCA with bradycardia induced by intravenous injection of adenosine triphosphate disodium hydrate (ATP).

MATERIAL AND METHODS:

SRCA was performed at the Photon Factory of the High Energy Accelerator Research Organization (Tsukuba, Japan). Eight male Wistar rats were anesthetized. A catheter for injecting the contrast material was inserted into the carotid artery. Temporary bradycardia was induced by an intravenous bolus injection of 5 mg of ATP, and SRCA was performed immediately thereafter.

RESULTS:

After ATP administration, the average heart rate decreased from 388 to 73 beats per minute. As a result, we could detect a coronary artery as small as 45 µm in diameter.

CONCLUSION:

Our SRCA system which has a high resolution of 9 µm per pixel could detect a coronary artery as small as 45 µm in diameter in the in vivo rat.

• Authors: Hiroaki Sakamoto, Shonosuke Matsushita, Kazuyuki Hyodo, Chiho Tokunaga, Fujio Sato, Yuji Hiramatsu
• Journal: Acta radiologica (Stockholm, Sweden : 1987) (Acta Radiol) Vol. 56 Issue 9 Pg. 1105-7 (Sep 2015) ISSN: 1600-0455 [Electronic] England
• PMID: 25260415 (Publication Type: Journal Article)
• Copyright: © The Foundation Acta Radiologica 2014.

Chemical References

• Contrast Media
• iomeprol
• Adenosine Triphosphate
• Iopamidol

Topics

• Adenosine Triphosphate (pharmacology)
• Animals
• Bradycardia (chemically induced)
• Contrast Media (administration & dosage)
• Coronary Angiography (instrumentation, methods)
• Coronary Vessels (anatomy & histology)
• Iopamidol (administration & dosage, analogs & derivatives)
• Male
• Rats
• Rats, Wistar
• Synchrotrons