The Study of "Dihydropyrimidinase Related Proteins (DRPs)" Expression Changes Influence in Malignant Astrocytoma Brain Tumor.

Abstract

BACKGROUND:

Dihydropyrimidinase Related Proteins (DRPs) have known homologous to the Collapsing Response Mediator Proteins (CRMPs). The DRP gene family has comprised four members, DRP 1, 2, 3, and 4, all out of which have considered to be involved in axonal outgrowth and path-finding.

METHODS:

The protein has extracted from tumor, normal brain tissues, and then the protein purity has evaluated by Bradford test and spectrophotometric methods. In this study, proteins has separated by Two-Dimensional Gel (2DG) electrophoresis method and then spots have analyzed and compared using statistical data and specific software (Progenesis Same Spots).Spots have identified by pH isoelectric, molecular weights and data banks.

RESULTS:

The 2D gel has shown 800 spots totally. Two spots have reported for DRP2, and one spot has reported for DRP3 in the human brain proteome, that have differed in pH isoelectric, and Molecular Weights values.

CONCLUSION:

This protein family has involved in neuronal differentiation and axonal guidance, and abundantly influenced in the developing brain, but their expression persisted into adulthood. DRP2 has regulated by phosphorylation, Glycogen synthase kinase 3, regulate phosphorylation of DRP2 an inactive from, and induced neuronal polarity.

• Authors: Mehdi Pooladi, Mostafa Rezaei-Tavirani, Mehrdad Hashemi, Saeed Hesami-Tackallou, Solmaz Khaghani-Razi-Abad, Afshin Moradi, Ali Reza Zali, Masoumea Mousavi, Azadeh Rakhshan, Leila Firozi-Dalvand, Roghaye Omidi
• Journal: Iranian journal of cancer prevention (Iran J Cancer Prev) Vol. 7 Issue 3 Pg. 130-6 ( 2014) ISSN: 2008-2398 [Print] Iran
• PMID: 25250163 (Publication Type: Journal Article)