[The role of arecoline on hepatic insulin resistance in type 2 diabetes rats].

Abstract

OBJECTIVE:

To explore the effects of arecoline on hepatic insulin resistance in type 2 diabetes rats and to elucidate its possible mechanism.

METHODS:

Forty five Wistar rats were fed with high fructose diet for 12 weeks to induce type 2 diabetic rat model. rats were randomly divided into 5 groups (n = 8): control group, model group and model group were treated with different dose (0, 0.5, 1, 5 mg/kg) of arecoline. After 4 weeks, the fasting blood glucose, blood lipid and insulin level measured , mRNA expression of liver constitutive androstane receptor (CAR), pregnane X receptor (PXR), glucose-6-phosphatase (G6Pase), phosphoenolpyruvate carboxykinase (PEPCK), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) were detected by reverse transcription polymerase chain reaction (RT-PCR), the protein expression of p-AKT and glucose transporter4 (GLUT4) were detected by Western blot.

RESULTS:

1.5 mg/kg arecoline could significantly decrease the level of fasting blood glucose, blood lipid, blood insulin level and liver G6Pase, PEPCK, IL-6, TNF-alpha mRNA level in type 2 diabetes rats. 1.5 mg/kg arecoline also could significantly increase CAR, PXR mRNA level and p-AKT and GLUT4 protein expression.

CONCLUSION:

Arecoline improved hepatic insulin resistance in type 2 diabetes rats by increasing the mRNA levels of CAR and PXR leading to the creased glucose metabolism and inflammation related genes expression.

• Authors: Hong-Yan Ling, Qi-Xin Yao, Zhu-Qing Qi, Si-Si Yang, Jian-Qin He, Kai-Fang Zhang, Bi Hu
• Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (Zhongguo Ying Yong Sheng Li Xue Za Zhi) Vol. 30 Issue 3 Pg. 208-12 (May 2014) ISSN: 1000-6834 [Print] China
• PMID: 25244782 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Constitutive Androstane Receptor
• Glucose Transporter Type 4
• Interleukin-6
• Intracellular Signaling Peptides and Proteins
• Pregnane X Receptor
• Receptors, Cytoplasmic and Nuclear
• Receptors, Steroid
• Slc2a4 protein, rat
• Tumor Necrosis Factor-alpha
• Arecoline
• Proto-Oncogene Proteins c-akt
• Glucose-6-Phosphatase
• Pck1 protein, rat
• Phosphoenolpyruvate Carboxykinase (GTP)

Topics

• Animals
• Arecoline (pharmacology)
• Constitutive Androstane Receptor
• Diabetes Mellitus, Experimental (metabolism)
• Diabetes Mellitus, Type 2 (metabolism)
• Glucose Transporter Type 4 (metabolism)
• Glucose-6-Phosphatase (metabolism)
• Insulin Resistance
• Interleukin-6 (metabolism)
• Intracellular Signaling Peptides and Proteins (metabolism)
• Liver (drug effects, metabolism)
• Male
• Phosphoenolpyruvate Carboxykinase (GTP) (metabolism)
• Pregnane X Receptor
• Proto-Oncogene Proteins c-akt (metabolism)
• Rats
• Rats, Wistar
• Receptors, Cytoplasmic and Nuclear (metabolism)
• Receptors, Steroid (metabolism)
• Tumor Necrosis Factor-alpha (metabolism)