Abstract | OBJECTIVES: METHODS: Flow cytometry was performed on 29 newly diagnosed APLs and 93 other acute myeloid leukemias, including 25 HLA-DR- or CD34- cases. Clinical notes from referring institutions were reviewed to assess for recent ATRA administration. RESULTS: Recent ATRA therapy was documented in 17 (59%) of 29 patients with APL. The main features of untreated APL were preserved with ATRA therapy, including CD34- (83% vs 82%), HLA-DR- (83% vs 100%), and CD117+ (100% vs 77%). CD11b and CD11c were negative in all untreated APLs but positive in 76% and 88% of ATRA-treated APLs, respectively. Optimal diagnostic criteria for untreated APL (CD34- or HLA-DR- and CD11b- and CD11c-) showed 100% sensitivity and 98% specificity but were not useful after ATRA administration. The best interpretative approach to ATRA-treated APL (CD34- or HLA-DR-) showed 100% sensitivity but limited specificity (73%). CONCLUSIONS: Information about recent ATRA administration is critical for adequate interpretation of the flow cytometric findings in patients with suspected APL.
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Authors | Pedro Horna, Ling Zhang, Eduardo M Sotomayor, Jeffrey E Lancet, Lynn C Moscinski |
Journal | American journal of clinical pathology
(Am J Clin Pathol)
Vol. 142
Issue 4
Pg. 546-52
(Oct 2014)
ISSN: 1943-7722 [Electronic] England |
PMID | 25239423
(Publication Type: Evaluation Study, Journal Article)
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Copyright | Copyright© by the American Society for Clinical Pathology. |
Chemical References |
- Antigens, CD
- Antigens, CD34
- Antigens, Neoplasm
- Antineoplastic Agents
- HLA-DR Antigens
- Tretinoin
|
Topics |
- Adult
- Aged
- Aged, 80 and over
- Antigens, CD
(analysis)
- Antigens, CD34
(analysis)
- Antigens, Neoplasm
(analysis)
- Antineoplastic Agents
(administration & dosage)
- Biopsy, Needle
- Bone Marrow
(pathology)
- Female
- Flow Cytometry
- Gene Expression Regulation, Neoplastic
- HLA-DR Antigens
(analysis)
- Humans
- Immunophenotyping
(methods)
- Leukemia, Promyelocytic, Acute
(diagnosis, drug therapy, immunology)
- Male
- Middle Aged
- Retrospective Studies
- Tretinoin
(administration & dosage)
- Young Adult
|