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Intranasal vaccination with extracellular serine proteases of Leishmania amazonensis confers protective immunity to BALB/c mice against infection.

AbstractBACKGROUND:
Previously, we demonstrated that unlike subcutaneous or intramuscular vaccination, intranasal vaccination of BALB/c mice with whole Leishmania amazonensis antigens leads to protection against cutaneous leishmaniasis. Here, the role of parasite serine proteases in the protective immunity was investigated.
FINDINGS:
Serine Proteases were partially purified from both soluble (LaSP-Sol) and extracellular (LaSP-Ex) Leishmania amazonensis promastigote extracts by aprotinin-agarose chromatography. BALB/c mice were intranasally immunized with LaSP-Sol and LaSP-Ex prior to infection with L. amazonensis. LaSP-Ex but not LaSP-Sol vaccination led to significantly smaller lesions and parasite burdens as compared with non-vaccinated controls. Protection was accompanied by systemic Th1 polarization with increased IFN-γ and decreased IL-4 and IL-10 splenic production. Likewise, increased production of IFN-γ, IL-12 and IL-4 concomitant with decreased TGF-β and TNF-α was locally observed in the infected footpad.
CONCLUSION:
This study indicates that extracellular serine proteases of L. amazonensis are strong candidates for a more defined intranasal vaccine against cutaneous leishmaniasis.
AuthorsHerbert Leonel de Matos Guedes, Beatriz Lilian da Silva Costa, Suzana Passos Chaves, Daniel Cláudio de Oliveira Gomes, Joshua Daniel Nosanchuk, Salvatore Giovanni De Simone, Bartira Rossi-Bergmann
JournalParasites & vectors (Parasit Vectors) Vol. 7 Pg. 448 ( 2014) ISSN: 1756-3305 [Electronic] England
PMID25239157 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References
  • Antigens, Protozoan
  • Cytokines
  • Protozoan Proteins
  • Protozoan Vaccines
  • Serine Proteases
Topics
  • Administration, Intranasal
  • Animals
  • Antigens, Protozoan (immunology)
  • Cytokines (metabolism)
  • Female
  • Leishmania mexicana (enzymology, immunology)
  • Leishmaniasis, Cutaneous (prevention & control)
  • Mice
  • Mice, Inbred BALB C
  • Protozoan Proteins (immunology)
  • Protozoan Vaccines (administration & dosage)
  • Serine Proteases (immunology)
  • Vaccination

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