[Effect of bear bile powder on STAT3 pathway in hepatocellular carcinoma xenograft].

Abstract

OBJECTIVE:

To observe the effect of bear bile powder (BBP) on the STAT3 pathway and its downstream target genes of nude mice hepatocellular carcinoma (HCC) xenograft, and to explore its mechanism for treating HCC.

METHODS:

The subcutaneous xenograft model was established using HepG2 cells. When the subcutaneous transplanted tumor was formed, naked mice were randomly divided into two groups, the BBP group and the control group. Mice in the BBP group were administered with BBP by gastrogavage, once daily for 3 consecutive weeks, while mice in the control group were administered with normal saline by gastrogavage, once daily for 3 consecutive weeks. The body weight and the tumor volume were measured once per week. By the end of medication, the tumor weight was weighed and the tumor inhibition ratio calculated. The apoptosis of the tumor tissue was detected by TdT-mediated dUTP nick end labeling (TUNEL). The expression of Bcl2-associated X protein (Bax), B cell lymphoma/eukemina-2 (Bcl-2), cyclin-dependent protein kinase (CDK4), cyclinD1 were detected by reverse transcription-polymerase chain reaction (RT-PCR). The protein expression levels of signal transducers and transcription activators 3 (p-STAT3), proliferating cell nuclear antigen (PCNA), Bax, Bcl-2, CDK4, and cyclinD1 were determined by immunohistochemistry.

RESULTS:

BBP could inhibit the tumor volume and tumor weight, showing statistical difference when compared with the control group (P < 0.01). Results of TUNEL showed that BBP could significantly induce the apoptosis of hepatoma carcinoma cells. Results of RT-PCR showed that BBP could up-regulate the expression of Bax and down-regulate mRNA expression of Bcl-2, CDK4, and cyclinD1. Immunohistochemical results showed that BBP could up-regulate the expression of Bax and inhibit the protein expression of p-STAT3, PCNA, Bcl-2, CDK4, and cyclinD1.

CONCLUSION:

BBP could induce the apoptosis of hepatoma carcinoma cells and inhibit their proliferation by regulating STAT3 pathway.

• Authors: Jin-Yan Zhao, Li-Ya Liu, A-Ling Shen, Wei Lin, Zhi-Yun Cao, Qun-Chuan Zhuang, Zhen-Feng Hong
• Journal: Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine (Zhongguo Zhong Xi Yi Jie He Za Zhi) Vol. 34 Issue 8 Pg. 976-81 (Aug 2014) ISSN: 1003-5370 [Print] China
• PMID: 25223184 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Drugs, Chinese Herbal
• Proto-Oncogene Proteins c-bcl-2
• STAT3 Transcription Factor
• bcl-2-Associated X Protein
• Cyclin D1
• Cyclin-Dependent Kinase 4

Topics

• Animals
• Bile
• Carcinoma, Hepatocellular (metabolism, pathology)
• Cyclin D1 (metabolism)
• Cyclin-Dependent Kinase 4 (metabolism)
• Drugs, Chinese Herbal (pharmacology)
• Hep G2 Cells
• Humans
• Liver Neoplasms (metabolism, pathology)
• Male
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• STAT3 Transcription Factor (metabolism)
• Signal Transduction
• Ursidae
• Xenograft Model Antitumor Assays
• bcl-2-Associated X Protein (metabolism)