Abstract | BACKGROUND: METHODS: Rats were randomized into four groups: Control; VDD; ischemia/reperfusion injury (IRI); and VDD+IRI. At the 62 day after sham or IRI surgery, we measured inulin clearance, biochemical variables and hemodynamic parameters. In kidney tissue, we performed immunoblotting to quantify expression of Klotho, TGF-β, and vitamin D receptor (VDR); gene expression to evaluate renin, angiotensinogen, and angiotensin-converting enzyme; and immunohistochemical staining for ED1 (macrophages), type IV collagen, fibronectin, vimentin, and α-smooth mucle actin. Histomorphometric studies were performed to evaluate fractional interstitial area. RESULTS: IRI animals presented renal hypertrophy, increased levels of mean blood pressure and plasma PTH. Furthermore, expansion of the interstitial area, increased infiltration of ED1 cells, increased expression of collagen IV, fibronectin, vimentin and α-actin, and reduced expression of Klotho protein were observed. VDD deficiency contributed to increased levels of plasma PTH as well as for important chronic tubulointerstitial changes ( fibrosis, inflammatory infiltration, tubular dilation and atrophy), increased expression of TGF-β1 and decreased expression of VDR and Klotho protein observed in VDD+IRI animals. CONCLUSION:
|
Authors | Janaína Garcia Gonçalves, Ana Carolina de Bragança, Daniele Canale, Maria Heloisa Massola Shimizu, Talita Rojas Sanches, Rosa Maria Affonso Moysés, Lúcia Andrade, Antonio Carlos Seguro, Rildo Aparecido Volpini |
Journal | PloS one
(PLoS One)
Vol. 9
Issue 9
Pg. e107228
( 2014)
ISSN: 1932-6203 [Electronic] United States |
PMID | 25222475
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Actins
- Fibronectins
- Receptors, Calcitriol
- Transforming Growth Factor beta
- Transforming Growth Factor beta1
- Angiotensinogen
- Renin
|
Topics |
- Actins
(metabolism)
- Acute Kidney Injury
- Angiotensinogen
(metabolism)
- Animals
- Disease Progression
- Fibronectins
(metabolism)
- Kidney
- Male
- Rats
- Rats, Wistar
- Receptors, Calcitriol
(metabolism)
- Renal Insufficiency, Chronic
(etiology, metabolism, pathology)
- Renin
(metabolism)
- Reperfusion Injury
(etiology, metabolism, pathology)
- Transforming Growth Factor beta
- Transforming Growth Factor beta1
(metabolism)
- Vitamin D Deficiency
(complications, metabolism, physiopathology)
|