Cardioprotection against ischemia/reperfusion by licochalcone B in isolated rat hearts.

Abstract

The generation of reactive oxygen species (ROS) is a major cause of heart injury induced by ischemia-reperfusion. The left ventricular developed pressure (LVDP) and the maximum up/down rate of left ventricular pressure (±dp/dt(max)) were documented by a physiological recorder. Myocardial infarct size was estimated macroscopically using 2,3,5-triphenyltetrazolium chloride staining. Coronary effluent was analyzed for lactate dehydrogenase (LDH) and creatine kinase (CK) release to assess the degree of cardiac injury. The levels of C-reactive protein (CRP), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) were analyzed to determine the inflammation status of the myocardial tissue. Cardiomyocyte apoptosis analysis was performed using the In Situ Cell Death Detection Kit, POD. Accordingly, licochalcone B pretreatment improved the heart rate (HR), increased LVDP, and decreased CK and LDH levels in coronary flow. SOD level and GSH/GSSG ratio increased, whereas the levels of MDA, TNF-α, and CRP and activities of IL-8 and IL-6 decreased in licochalcone B-treated groups. The infarct size and cell apoptosis in hearts from licochalcone B-treated group were lower than those in hearts from the I/R control group. Therefore, the cardioprotective effects of licochalcone B may be attributed to its antioxidant, antiapoptotic, and anti-inflammatory activities.

• Authors: Jichun Han, Dong Wang, Bacui Yu, Yanming Wang, Huanhuan Ren, Bo Zhang, Yonghua Wang, Qiusheng Zheng
• Journal: Oxidative medicine and cellular longevity (Oxid Med Cell Longev) Vol. 2014 Pg. 134862 ( 2014) ISSN: 1942-0994 [Electronic] United States
• PMID: 25215172 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cardiotonic Agents
• Chalcones
• Interleukin-6
• Interleukin-8
• Tumor Necrosis Factor-alpha
• C-Reactive Protein
• L-Lactate Dehydrogenase
• Superoxide Dismutase
• Creatine Kinase
• licochalcone B
• Glutathione

Topics

• Animals
• Apoptosis
• C-Reactive Protein (metabolism)
• Cardiotonic Agents (pharmacology, therapeutic use)
• Chalcones (pharmacology, therapeutic use)
• Creatine Kinase (metabolism)
• Glutathione (metabolism)
• Heart (drug effects)
• In Vitro Techniques
• Interleukin-6 (metabolism)
• Interleukin-8
• L-Lactate Dehydrogenase (metabolism)
• Male
• Myocardial Reperfusion Injury (physiopathology, prevention & control)
• Myocardium (metabolism, pathology)
• Oxidative Stress
• Rats
• Rats, Sprague-Dawley
• Superoxide Dismutase (metabolism)
• Tumor Necrosis Factor-alpha (metabolism)