[Two-dimensional zebrafish model combined with hyphenated chromatographic techniques for evaluation anti-osteoporosis activity of epimendin A and its metabolite baohuoside I].

Abstract

This article firstly established a new efficient method for screening anti-osteoporosis ingredients, which used two-dimensional zebrafish model combined with hyphenated chromatographic techniques to evaluate anti-osteoporosis activities of epimedin A and its metabolite baohuoside I. Adult zebrafish was used for metabolism of epimedin A in 0.5% DMSO, and LC-MS was used for analysis of the metabolite, which was captured by HPLC, and prednisolone-induced osteoporosis model of zebrafish was used to evaluate the anti-osteoporotic activities of trace amounts of epimedin A and baohuoside I. The results indicated that epimedin A and baohuoside I can prevent prednisolone-induced osteoporosis in zebrafish. The developed method in this paper enables the separation, enrichment and analysis of micro-amount metabolite of epimedin A, and anti-osteoporosis activities in vivo of epimedin A and baohuoside I was simple and efficient screening resorting to zebrafish osteoporosis mode. This paper would provide new ideas and methods for a rapid and early discovery of anti-osteoporosis activities of micro-ingredients and its metabolite of traditional Chinese medicine.

• Authors: Yang Zhan, Ying-Jie Wei, E Sun, Feng-Juan Xu, Xiao-Bin Jia
• Journal: Yao xue xue bao = Acta pharmaceutica Sinica (Yao Xue Xue Bao) Vol. 49 Issue 6 Pg. 932-7 (Jun 2014) ISSN: 0513-4870 [Print] China
• PMID: 25212043 (Publication Type: Journal Article)

Chemical References

• Drugs, Chinese Herbal
• Flavonoids
• epimedin A
• baohuoside I

Topics

• Animals
• Chromatography, High Pressure Liquid
• Chromatography, Liquid
• Drug Evaluation, Preclinical
• Drugs, Chinese Herbal (pharmacology)
• Flavonoids (metabolism, pharmacology)
• Mass Spectrometry
• Osteoporosis (drug therapy)
• Zebrafish