Quantitative proteomics study of protective effects of grape seed procyanidin B2 on diabetic cardiomyopathy in db/db mice.

Abstract

Diabetic cardiomyopathy is one of the major complications of diabetes mellitus. Oxidative stress appears to play a substantial role in cardiomyopathy. Grape seed procyanidin B2 (GSPB2) has been known as an anti-oxidant in treating diabetes mellitus; however, little is known about its effects and underlying mechanisms on diabetic cardiomyopathy. The present study is to explore the molecular targets of GSPB2 responsible for the anti-oxidative effects in db/db mice by quantitative proteomics. GSPB2 (30 mg/kg body weight/day) were intragastric administrated to db/db mice for 10 weeks. Proteomics of the heart tissue extracts by isobaric tags for relative and absolute quantification analysis was obtained from db/db mice. Our study provides important evidence that GSPB2 protect against cardiomyopathy in diabetes mellitus, which are believed to result from regulating the expression of key proteins involving cardiac fibrosis and proliferation. GSPB2 could be expected to become novel clinical application in fighting against diabetic cardiomyopathy.

• Authors: Si-si Luan, Fei Yu, Bao-ying Li, Rui-jie Qin, Xiao-li Li, Qian Cai, Wen-bin Yin, Mei Cheng, Hai-qing Gao
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 78 Issue 9 Pg. 1577-83 ( 2014) ISSN: 1347-6947 [Electronic] England
• PMID: 25209507 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Biflavonoids
• Grape Seed Extract
• Proanthocyanidins
• Protective Agents
• procyanidin B2
• Catechin

Topics

• Animals
• Antioxidants (administration & dosage, metabolism)
• Apoptosis (drug effects)
• Biflavonoids (administration & dosage)
• Catechin (administration & dosage)
• Diabetic Cardiomyopathies (drug therapy, metabolism, pathology)
• Evaluation Studies as Topic
• Grape Seed Extract (chemistry)
• Male
• Mice
• Oxidative Stress (drug effects)
• Proanthocyanidins (administration & dosage)
• Protective Agents (administration & dosage, chemistry)
• Proteomics