Gene expression changes in progression of cervical neoplasia revealed by microarray analysis of cervical neoplastic keratinocytes.

Abstract

To evaluate the gene expression changes involved in neoplastic progression of cervical intraepithelial neoplasia. Using microarray analysis, large-scale gene expression profile was carried out on HPV16-CIN2, HPV16-CIN3, and normal cervical keratinocytes derived from two HPV16-CIN2, two HPV-CIN3 lesions, and two corresponding normal cervical tissues, respectively. Differentially expressed genes were analyzed in normal cervical keratinocytes compared with HPV16-CIN2 keratinocytes and in HPV16-CIN2 keratinocytes compared with HPV16-CIN3 keratinocytes; 37 candidate genes with continuously increasing or decreasing expression during CIN progression were identified. One of these genes, phosphoglycerate dehydrogenase, was chosen for further characterization. Quantitative reverse transcription-polymerase chain reaction and immunohistochemical analysis confirmed that expression of phosphoglycerate dehydrogenase consistently increases during progression of CIN toward cancer. Gene expression changes occurring during CIN progression were investigated using microarray analysis, for the first time, in CIN2 and CIN3 keratinocytes naturally infected with HPV16. Phosphoglycerate dehydrogenase is likely to be associated with tumorigenesis and may be a potential prognostic marker for CIN progression.

• Authors: John Charles Rotondo, Silvia Bosi, Cristian Bassi, Manuela Ferracin, Giovanni Lanza, Roberta Gafà, Eros Magri, Rita Selvatici, Stefania Torresani, Roberto Marci, Paola Garutti, Massimo Negrini, Mauro Tognon, Fernanda Martini
• Journal: Journal of cellular physiology (J Cell Physiol) Vol. 230 Issue 4 Pg. 806-12 (Apr 2015) ISSN: 1097-4652 [Electronic] United States
• PMID: 25205602 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 Wiley Periodicals, Inc.

Chemical References

• Biomarkers, Tumor
• Phosphoglycerate Dehydrogenase

Topics

• Biomarkers, Tumor (genetics, metabolism)
• Carcinoma, Squamous Cell (pathology)
• Cell Transformation, Neoplastic (genetics)
• Disease Progression
• Female
• Gene Expression Regulation, Neoplastic (genetics)
• Human papillomavirus 16 (isolation & purification)
• Humans
• Keratinocytes (metabolism)
• Papillomavirus Infections (genetics)
• Phosphoglycerate Dehydrogenase (genetics, metabolism)
• Tissue Array Analysis
• Uterine Cervical Neoplasms (genetics, pathology)
• Uterine Cervical Dysplasia (genetics)