Argonaute high-throughput sequencing of RNAs isolated by cross-linking immunoprecipitation reveals a snapshot of miRNA gene regulation in the mammalian retina.

Abstract

Mounting evidence points to roles for miRNA gene regulation in promoting development, function, and cell survival in the mammalian retina. However, little is known regarding which retinal genes are targets of miRNAs. Here, we employed a systematic, nonbiased, biochemical approach to identify targets of miRNA gene regulation in the bovine retina, a common model species for vision research. Using Argonaute high-throughput sequencing of RNAs isolated by cross-linking immunoprecipitation analysis, we identified 348 high-confidence miRNA target sites within 261 genes. This list was enriched in rod and cone photoreceptor genes and included 28 retinal disease genes, providing further evidence of a role of miRNAs in the pathology of blinding diseases.

• Authors: Thomas R Sundermeier, Hui Jin, Matthew L Kleinjan, Debarshi Mustafi, Donny D Licatalosi, Krzysztof Palczewski
• Journal: Biochemistry (Biochemistry) Vol. 53 Issue 37 Pg. 5831-3 (Sep 23 2014) ISSN: 1520-4995 [Electronic] United States
• PMID: 25204418 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Argonaute Proteins
• MicroRNAs

Topics

• Animals
• Argonaute Proteins (immunology)
• Cattle
• Gene Expression Regulation
• High-Throughput Nucleotide Sequencing (methods)
• Immunoprecipitation (methods)
• MicroRNAs (genetics)
• Retina (physiology)