Abstract |
Glaucoma is a leading cause of blindness, afflicting more than 60 million people worldwide. Increased intraocular pressure (IOP) due to impaired aqueous humor drainage is a major risk factor for the development of glaucoma. Here, we demonstrated that genetic disruption of the angiopoietin/TIE2 (ANGPT/TIE2) signaling pathway results in high IOP, buphthalmos, and classic features of glaucoma, including retinal ganglion degeneration and vision loss. Eyes from mice with induced deletion of Angpt1 and Angpt2 (A1A2Flox(WB) mice) lacked drainage pathways in the corneal limbus, including Schlemm's canal and lymphatic capillaries, which share expression of the PROX1, VEGFR3, and FOXC family of transcription factors. VEGFR3 and FOXCs have been linked to lymphatic disorders in patients, and FOXC1 has been linked to glaucoma. In contrast to blood endothelium, in which ANGPT2 is an antagonist of ANGPT1, we have shown that both ligands cooperate to regulate TIE2 in the lymphatic network of the eye. While A1A2Flox(WB) mice developed high IOP and glaucoma, expression of ANGPT1 or ANGPT2 alone was sufficient for ocular drainage. Furthermore, we demonstrated that loss of FOXC2 from lymphatics results in TIE2 downregulation, suggesting a mechanism for ocular defects in patients with FOXC mutations. These data reveal a pathogenetic and molecular basis for glaucoma and demonstrate the importance of angiopoietin ligand cooperation in the lymphatic endothelium.
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Authors | Benjamin R Thomson, Stefan Heinen, Marie Jeansson, Asish K Ghosh, Anees Fatima, Hoon-Ki Sung, Tuncer Onay, Hui Chen, Shinji Yamaguchi, Aris N Economides, Ann Flenniken, Nicholas W Gale, Young-Kwon Hong, Amani Fawzi, Xiaorong Liu, Tsutomu Kume, Susan E Quaggin |
Journal | The Journal of clinical investigation
(J Clin Invest)
Vol. 124
Issue 10
Pg. 4320-4
(Oct 2014)
ISSN: 1558-8238 [Electronic] United States |
PMID | 25202984
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Angiopoietin-1
- Angiopoietin-2
- Forkhead Transcription Factors
- Foxc1 protein, mouse
- Homeodomain Proteins
- Ligands
- Tumor Suppressor Proteins
- prospero-related homeobox 1 protein
- Receptor, TIE-2
- Tek protein, mouse
- Vascular Endothelial Growth Factor Receptor-3
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Topics |
- Angiopoietin-1
(genetics)
- Angiopoietin-2
(genetics)
- Animals
- Aqueous Humor
- Cell Separation
- Disease Models, Animal
- Down-Regulation
- Flow Cytometry
- Forkhead Transcription Factors
(metabolism)
- Glaucoma
(pathology)
- Homeodomain Proteins
(metabolism)
- Intraocular Pressure
- Ligands
- Lymphatic System
(pathology)
- Mice
- Mice, Knockout
- Mutation
- Ocular Hypertension
(pathology)
- Receptor, TIE-2
(genetics)
- Trabecular Meshwork
(metabolism)
- Tumor Suppressor Proteins
(metabolism)
- Vascular Endothelial Growth Factor Receptor-3
(metabolism)
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