Abstract |
In human pathogenic bacteria, nickel is required for the activation of two enzymes, urease and [ NiFe]-hydrogenase, necessary for host infection. Acquisition of Ni(II) is mediated by either permeases or ABC-importers, the latter including a subclass that involves an extracytoplasmic nickel- binding protein, Ni-BP. This study reports on the structure of three Ni-BPs from a diversity of human pathogens and on the existence of three new nickel-binding motifs. These are different from that previously described for Escherichia coli Ni-BP NikA, known to bind nickel via a nickelophore, and indicate a variegated ligand selectivity for Ni-BPs. The structures are consistent with ligand affinities measured in solution by calorimetry and challenge the hypothesis of a general requirement of nickelophores for nickel uptake by canonical ABC importers. Phylogenetic analyses showed that Ni-BPs have different evolutionary origins and emerged independently from peptide- binding proteins, possibly explaining the promiscuous behavior of this class of Ni(II) carriers.
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Authors | Hugo Lebrette, Céline Brochier-Armanet, Barbara Zambelli, Hilde de Reuse, Elise Borezée-Durant, Stefano Ciurli, Christine Cavazza |
Journal | Structure (London, England : 1993)
(Structure)
Vol. 22
Issue 10
Pg. 1421-32
(Oct 07 2014)
ISSN: 1878-4186 [Electronic] United States |
PMID | 25199691
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier Ltd. All rights reserved. |
Chemical References |
- Bacterial Proteins
- Carrier Proteins
- Nickel
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Topics |
- Amino Acid Motifs
- Amino Acid Sequence
- Bacterial Proteins
(chemistry, metabolism)
- Binding Sites
- Biological Transport
- Brucella suis
(chemistry, pathogenicity)
- Campylobacter jejuni
(chemistry, pathogenicity)
- Carrier Proteins
(chemistry, metabolism)
- Crystallography, X-Ray
- Evolution, Molecular
- Models, Molecular
- Molecular Sequence Data
- Nickel
(metabolism)
- Phylogeny
- Protein Conformation
- Thermodynamics
- Yersinia pestis
(chemistry, pathogenicity)
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