Induction of potential protective immunity against enterotoxemia in calves by single or multiple recombinant Clostridium perfringens toxoids.

Abstract

Cattle enterotoxemia caused by Clostridium perfringens toxins is a noncontagious, sporadic, and fatal disease characterized by sudden death. Strategies for controlling and preventing cattle enterotoxemia are based on systematic vaccination of herds with toxoids. Because the process of producing conventional clostridial vaccines is dangerous, expensive, and time-consuming, the prospect of recombinant toxoid vaccines against diseases caused by C. perfringens toxins is promising. In this study, nontoxic recombinant toxoids derived from α-, β- and ε-toxins of C. perfringens, namely, rCPA247-370 , rCPB and rEtxHP, respectively, were expressed in Escherichia coli. High levels of specific IgG antibodies and neutralizing antibodies against the toxins were detected in sera from calves vaccinated with either a single recombinant toxoid or a mixed cocktail of all three recombinant toxoids, indicating the potential of these recombinant toxoids to provide calves with protective immunity against enterotoxemia caused by C. perfringens.

• Authors: Zhigang Jiang, Yanyan De, Jitao Chang, Fang Wang, Li Yu
• Journal: Microbiology and immunology (Microbiol Immunol) Vol. 58 Issue 11 Pg. 621-7 (Nov 2014) ISSN: 1348-0421 [Electronic] Australia
• PMID: 25197030 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 The Societies and Wiley Publishing Asia Pty Ltd.

Chemical References

• Antibodies, Bacterial
• Antibodies, Neutralizing
• Antitoxins
• Clostridium perfringens toxoid
• Immunoglobulin G
• Recombinant Proteins
• Toxoids

Topics

• Animals
• Antibodies, Bacterial (blood)
• Antibodies, Neutralizing (blood)
• Antitoxins (blood)
• Cattle
• Cattle Diseases (prevention & control)
• Clostridium Infections (prevention & control, veterinary)
• Clostridium perfringens (genetics, immunology)
• Enterotoxemia (prevention & control)
• Escherichia coli (genetics)
• Female
• Gene Expression
• Immunoglobulin G (blood)
• Recombinant Proteins (administration & dosage, genetics, immunology, isolation & purification)
• Toxoids (administration & dosage, genetics, immunology, isolation & purification)