Phytochemical profile of a microalgae Euglena tuba and its hepatoprotective effect against iron-induced liver damage in Swiss albino mice.

Abstract

AIMS:

This study was aimed to evaluate different phytochemical constituents and the ameliorating effect of 70% methanol extract of Euglena tuba (ETME) on iron overload-induced liver injury, along with its in vitro iron-chelating and DNA protection effects.

METHODS AND RESULTS:

Phytochemicals of ETME were identified by GC-MS analysis. Iron chelation and protection of Fenton reaction-induced DNA damage was conducted in vitro. Post oral administration of ETME to iron-overloaded mice, the levels of serum parameters, antioxidant enzymes, liver iron, lipid peroxidation, protein carbonyl and hydroxyproline contents were measured. ETME showed inhibition of lipid peroxidation, protein oxidation and liver fibrosis. The serum markers and liver iron were lessened, whereas enhanced levels of liver antioxidant enzymes were detected in ETME-treated group. Furthermore, the histopathological observations also substantiated the protective effects of the extract.

CONCLUSIONS:

Several bioactive compounds identified by GC-MS may be the basis of hepatoprotective as well as antioxidant and iron-chelating effect of ETME.

SIGNIFICANCE AND IMPACT OF THE STUDY:

Currently available iron-chelating agents show several side effects and limitations which may be overcome by ETME, which suggest its benefit against pathology of iron overload-linked diseases. Hence, ETME can be used as a promising hepatoprotective agent.

• Authors: S Panja, D Chaudhuri, N B Ghate, N Mandal
• Journal: Journal of applied microbiology (J Appl Microbiol) Vol. 117 Issue 6 Pg. 1773-86 (Dec 2014) ISSN: 1365-2672 [Electronic] England
• PMID: 25195957 (Publication Type: Journal Article)
• Copyright: © 2014 The Society for Applied Microbiology.

Chemical References

• Antioxidants
• Cell Extracts
• Iron Chelating Agents
• Phytochemicals
• Protective Agents
• Iron

Topics

• Animals
• Antioxidants (metabolism)
• Cell Extracts (chemistry, therapeutic use)
• Euglena (chemistry)
• Iron (metabolism)
• Iron Chelating Agents (therapeutic use)
• Iron Overload (drug therapy, enzymology, metabolism, pathology)
• Lipid Peroxidation
• Liver (drug effects, metabolism, pathology)
• Male
• Mice
• Microalgae (chemistry)
• Oxidation-Reduction
• Phytochemicals (chemistry)
• Protective Agents (therapeutic use)