Cloning, purification and preliminary crystallographic analysis of the Helicobacter pylori pseudaminic acid biosynthesis N-acetyltransferase PseH.

Abstract

Helicobacter pylori infection is the common cause of gastritis and duodenal and stomach ulcers, which have been linked to a higher risk of the development of gastric cancer. The motility that facilitates persistent infection requires functional flagella that are heavily glycosylated with 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-nonulosonic acid (pseudaminic acid). Pseudaminic acid biosynthesis protein H (PseH) catalyzes the third step in its biosynthetic pathway, producing UDP-2,4-diacetamido-2,4,6-trideoxy-β-L-altropyranose. Crystals of H. pylori PseH have been grown by the hanging-drop vapour-diffusion method using diammonium tartrate as a precipitating agent. The crystals belonged to space group I222 or I212121, with unit-cell parameters a = 107.8, b = 145.4, c = 166.3 Å. A complete X-ray diffraction data set has been collected to 2.5 Å resolution using cryocooling conditions and synchrotron radiation.

• Authors: Yu C Liu, Abu I Ud-Din, Anna Roujeinikova
• Journal: Acta crystallographica. Section F, Structural biology communications (Acta Crystallogr F Struct Biol Commun) Vol. 70 Issue Pt 9 Pg. 1276-9 (Sep 2014) ISSN: 2053-230X [Electronic] United States
• PMID: 25195909 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Proteins
• DNA Primers
• Acetyltransferases

Topics

• Acetyltransferases (chemistry, genetics, isolation & purification)
• Amino Acid Sequence
• Bacterial Proteins (chemistry, genetics, isolation & purification)
• Base Sequence
• Cloning, Molecular
• DNA Primers
• Helicobacter pylori (enzymology)
• Molecular Sequence Data
• Polymerase Chain Reaction