Abstract | BACKGROUND AND AIMS: METHODS: Time- and dose-dependent antiproliferative and apoptotic effects of quercitrin determined by WST-1 cell proliferation assay, lactate dehydrogenase (LDH) cytotoxicity assay, determination of nucleosome enrichment factor, changes in caspase-3 activity, loss of mitochondrial membrane potential ( MMP) and also the localization of phosphatidylserine in the plasma membrane. Changes in whole genome gene expression levels were examined by Illumina Human HT-12v4 beadchip microarrays. RESULTS: There were significant increases in caspase-3 activity, loss of MMP, and increases in apoptotic cell population in response to quercitrin in A549 and NCI-H358 NSCLC cells in a time- and dose-dependent manner. CONCLUSION: Our results demonstrated that genes involved in leukocyte transendothelial migration, cell adhesion and phosphatidylinositol signaling system pathways were the most statistically significant pathways in NCI-H358 and A549 cells. These results revealed that quercitrin has antiproliferative and apoptotic effects on lung cancer cells by modulating the immune response. After confirming its anticarcinogenic effects in vivo, quercitrin could be a novel and strong anticancer agent against NSCLC.
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Authors | Zeynep Birsu Cincin, Miray Unlu, Bayram Kiran, Elif Sinem Bireller, Yusuf Baran, Bedia Cakmakoglu |
Journal | Archives of medical research
(Arch Med Res)
Vol. 45
Issue 6
Pg. 445-54
(Aug 2014)
ISSN: 1873-5487 [Electronic] United States |
PMID | 25193878
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 IMSS. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Antineoplastic Agents
- Biomarkers
- quercitrin
- Quercetin
- Caspase 3
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Topics |
- Antineoplastic Agents
(pharmacology, therapeutic use)
- Apoptosis
(drug effects, genetics)
- Biomarkers
(metabolism)
- Carcinoma, Non-Small-Cell Lung
(drug therapy, enzymology, genetics)
- Caspase 3
(metabolism)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Dose-Response Relationship, Drug
- Gene Expression Regulation, Neoplastic
(drug effects)
- Humans
- Lung Neoplasms
(drug therapy, enzymology, genetics)
- Membrane Potential, Mitochondrial
(drug effects)
- Quercetin
(analogs & derivatives, pharmacology, therapeutic use)
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