Effects of TiO2 nano glass ionomer cements against normal and cancer oral cells.

Abstract

BACKGROUND/AIM:

Incorporation of nanoparticles (NPs) into the glass ionomer cements (GICs) is known to improve their mechanical and antibacterial properties. The present study aimed to investigate the possible cytotoxicity and pro-inflammation effect of three different powdered GICs (base, core build and restorative) prepared with and without titanium dioxide (TiO2) nanoparticles.

MATERIALS AND METHODS:

Each GIC was blended with TiO2 nanopowder, anatase phase, particle size <25 nm at 3% and 5% (w/w), and the GIC blocks of cements were prepared in a metal mold. The GICs/TiO2 nanoparticles cements were smashed up with a mortar and pestle to a fine powder, and then subjected to the sterilization by autoclaving. Human oral squamous cell carcinoma cell lines (HCS-2, HSC-3, HSC-4, Ca9-22) and human normal oral cells [gingival fibroblast (HGF), pulp (HPC) and periodontal ligament fibroblast (HPLF)] were incubated with different concentrations of GICs in the presence or absence of TiO2 nanoparticles, and the viable cell number was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Prostaglandin E2 was quantified by enzyme-linked immunosorbent assay (ELISA). Changes in fine cell structure were assessed by transmission electron microscopy.

RESULTS:

Cancer cells exhibited moderate cytotoxicity after 48 h of incubation, regardless of the type of GIC and the presence or absence of TiO2 NPs. GICs induced much lower cytotoxicity against normal cells, but induced prostaglandin E2 production, in a synergistic wanner with interleukin-1β.

CONCLUSION:

The present study shows acceptable to moderate biocompatibility of GICs impregnated with TiO2 nanoparticles, as well as its pro-inflammatory effects at higher concentrations.

• Authors: Rene Garcia-Contreras, Rogelio J Scougall-Vilchis, Rosalia Contreras-Bulnes, Yumiko Kanda, Hiroshi Nakajima, Hiroshi Sakagami
• Journal: In vivo (Athens, Greece) (In Vivo) 2014 Sep-Oct Vol. 28 Issue 5 Pg. 895-907 ISSN: 1791-7549 [Electronic] Greece
• PMID: 25189906 (Publication Type: Journal Article)
• Copyright: Copyright © 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

Chemical References

• Antineoplastic Agents
• Glass Ionomer Cements
• titanium dioxide
• Titanium
• Dinoprostone

Topics

• Antineoplastic Agents (chemistry, pharmacology, toxicity)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Dinoprostone (biosynthesis)
• Dose-Response Relationship, Drug
• Glass Ionomer Cements (chemistry, pharmacology, toxicity)
• Humans
• Mouth Neoplasms (metabolism, ultrastructure)
• Nanoparticles (administration & dosage, chemistry)
• Titanium (administration & dosage)