Ethylenediamine-N,N,N',N'-tetrakis (methylenephosphonic
acid) (
EDTPO) is a potent inhibitor of both formation and dissolution of
hydroxyapatite crystals in vitro as well as 1-hydroxyethylidene-1, 1-bisphosphonic
acid (HEBP). The purpose of this study was to compare with the
biological effects of the same molar level of
EDTPO, HEBP and ethylenediamintetraaceticacid (
EDTA), prototype of
EDTPO, on bone, incisor dentin and serum chemistry in young rats. HEBP at 8mg P/kg does chosen based on it's toxicity, and 16mg P/kg of
EDTPO and 48mg/kg of
EDTA which were identical molar level with 8mg P/kg of HEBP, were injected subcutaneously at once a day for 10 days. At the end of treatment period blood for chemical analysis, and tibia and mandibular for histological examination were taken. Results obtained were; 1)
EDTPO and HEBP impaired the net
weight gain during experimental period, by about 80% of final
body weight in control group, but
EDTA did not affect. 2) The biochemical findings of serum were as follows;
calcium (Ca) level increased in
EDTPO and HEBP group, and inorganic
phosphorus (IP) level decreased in
EDTPO and HEBP group when compared to control group, and changes of Ca and IP level in
EDTPO group was more remarkable than those of HEBP group. Alkalie
phosphatase level was decreased in
EDTPO group, but increased in HEBP group compared to control group. There were no significant differences between
EDTA group and control group, excepting for decrease of Ca level. 3) In histological aspects of the tibia,
EDTPO and HEBP groups revealed enlargement of growth plates, being greater in
EDTPO group than HEBP group, over the control group, and showed expanded hypertrophic zone of growth plates and wide osteoid seams in the metaphysis.
EDTPO group also inhibited the calcification of dentin of mandibular incisor more strongly than HEBP group. From these data, it was indicated that
EDTPO is more potent inhibitor of calcification than HEBP. Both of
EDTPO and HEBP group, moreover, revealed enlargement of tibial metaphysis. These phenomena suggest that
EDTPO also inhibits
bone resorption as well as HEBP.
EDTA group did not reveal any differences compared to control group.