Abstract | RATIONALE: Air pollution exposure has been shown to potentiate plaque progression in humans and animals. Our previous studies have suggested a role for oxidized lipids in mediating adverse vascular effect of air pollution. However, the types of oxidized lipids formed in response to air pollutants and how this occurs and their relevance to atherosclerosis are not fully understood. OBJECTIVE: METHODS AND RESULTS:
Atherosclerosis-prone ApoE(-/-) or LDLR(-/-) mice were exposed to filtered air or concentrated ambient PM2.5 using a versatile aerosol concentrator enrichment system for 6 months. PM2.5 increased 7-ketocholesterol (7-KCh), an oxidatively modified form of cholesterol, in plasma intermediate density lipoprotein/ low-density lipoprotein fraction and in aortic plaque concomitant with progression of atherosclerosis and increased CD36 expression in plaque macrophages from PM2.5-exposed mice. Macrophages isolated from PM2.5-exposed mice displayed increased uptake of oxidized lipids without alterations in their efflux capacity. Consistent with these finding, CD36-positive macrophages displayed a heightened capacity for oxidized lipid uptake. Deficiency of CD36 on hematopoietic cells diminished the effect of air pollution on 7-KCh accumulation, foam cell formation, and atherosclerosis. CONCLUSIONS: Our results suggest a potential role for CD36-mediated abnormal accumulations of oxidized lipids, such as 7-KCh, in air pollution-induced atherosclerosis progression.
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Authors | Xiaoquan Rao, Jixin Zhong, Andrei Maiseyeu, Bhavani Gopalakrishnan, Frederick A Villamena, Lung-Chi Chen, Jack R Harkema, Qinghua Sun, Sanjay Rajagopalan |
Journal | Circulation research
(Circ Res)
Vol. 115
Issue 9
Pg. 770-780
(Oct 10 2014)
ISSN: 1524-4571 [Electronic] United States |
PMID | 25186795
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | © 2014 American Heart Association, Inc. |
Chemical References |
- Apolipoproteins E
- CD36 Antigens
- Ketocholesterols
- Particulate Matter
- Receptors, LDL
- 7-ketocholesterol
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Topics |
- Animals
- Aorta
(metabolism, pathology)
- Aortic Diseases
(blood, etiology, pathology)
- Apolipoproteins E
(deficiency, genetics)
- Atherosclerosis
(blood, etiology, pathology)
- Biological Transport
- CD36 Antigens
(blood, deficiency, genetics)
- Disease Models, Animal
- Disease Progression
- Foam Cells
(metabolism)
- Human Umbilical Vein Endothelial Cells
(metabolism)
- Humans
- Inhalation Exposure
- Ketocholesterols
(blood)
- Macrophages
(metabolism)
- Male
- Mice
- Mice, Knockout
- Oxidation-Reduction
- Particle Size
- Particulate Matter
- Plaque, Atherosclerotic
- Receptors, LDL
(deficiency, genetics)
- Time Factors
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