Naringin prevents ovariectomy-induced osteoporosis and promotes osteoclasts apoptosis through the mitochondria-mediated apoptosis pathway.

Abstract

Naringin, the primary active compound of the traditional Chinese medicine Rhizoma drynariae, possesses many pharmacological activities. The present study is an effort to explore the anti-osteoporosis potential of naringin in vivo and in vitro. In vivo, we used ovariectomized rats to clarify the mechanisms by which naringin anti-osteoporosis. In vitro, we used osteoclasts to investigate naringin promotes osteoclasts apoptosis. Naringin was effective at enhancing BMD, trabecular thickness, bone mineralization, and mechanical strength in a dose-dependent manner. The result of RT-PCR analysis revealed that naringin down-regulated the mRNA expression levels of BCL-2 and up-regulated BAX, caspase-3 and cytochrome C. In addition, naringin significantly reduced the bone resorption area in vitro. These findings suggest that naringin promotes the apoptosis of osteoclasts by regulating the activity of the mitochondrial apoptosis pathway and prevents OVX-induced osteoporosis in rats.

• Authors: Fengbo Li, Xiaolei Sun, Jianxiong Ma, Xinlong Ma, Bin Zhao, Yang Zhang, Peng Tian, Yanjun Li, Zhe Han
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 452 Issue 3 Pg. 629-35 (Sep 26 2014) ISSN: 1090-2104 [Electronic] United States
• PMID: 25181344 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Bone Density Conservation Agents
• Flavanones
• Plant Extracts
• Proto-Oncogene Proteins c-bcl-2
• Cytochromes c
• Caspase 3
• naringin

Topics

• Animals
• Apoptosis (drug effects)
• Bone Density (drug effects)
• Bone Density Conservation Agents (isolation & purification, pharmacology)
• Bone Resorption (genetics, metabolism, pathology, prevention & control)
• Calcification, Physiologic (drug effects)
• Caspase 3 (genetics, metabolism)
• Cell Line
• Cytochromes c (genetics, metabolism)
• Dose-Response Relationship, Drug
• Female
• Flavanones (isolation & purification, pharmacology)
• Gene Expression (drug effects)
• Humans
• Mitochondria (drug effects, metabolism)
• Osteoclasts (drug effects, metabolism, pathology)
• Osteoporosis (etiology, genetics, metabolism, prevention & control)
• Ovariectomy (adverse effects)
• Plant Extracts (chemistry)
• Proto-Oncogene Proteins c-bcl-2 (genetics, metabolism)
• Rats
• Rats, Sprague-Dawley