The present investigation was an attempt to elucidate oxidative stress induced by
bisphenol A on erythrocytes and its amelioration by
green tea extract. For this, venous blood samples from healthy human adults were collected in
EDTA vials and used for preparation of erythrocytes
suspension. When erythrocyte
suspensions were treated with different concentrations of BPA/H2O2, a dose-dependent increase in
hemolysis occurred. Similarly, when erythrocytes
suspensions were treated with either different concentrations of H2O2 (0.05-0.25 mM) along with BPA (50 μg/mL) or 0.05 mM H2O2 along with different concentrations of BPA (50-250 μg/mL), dose-dependent significant increase in
hemolysis occurred. The effect of BPA and H2O2 was found to be additive. For the confirmation, binding capacity of
bisphenol A with erythrocyte
proteins (
hemoglobin,
catalase, and
glutathione peroxidase) was inspected using molecular docking tool, which showed presence of various hydrogen bonds of BPA with the
proteins. The present data clearly indicates that BPA causes oxidative stress in a similar way as H2O2 . Concurrent addition of different concentrations (10-50 μg/mL) of
green tea extract to reaction mixture containing high dose of
bisphenol A (250 μg/mL) caused concentration-dependent amelioration in
bisphenol A-induced
hemolysis. The effect was significant (P < 0.05). It is concluded that BPA-induced oxidative stress could be significantly mitigated by
green tea extract.