Abstract | OBJECTIVES: The aim of this study was to verify the promoting effect of carbamide peroxide on dimethylbenzanthracene (DMBA)-induced carcinogenesis in the hamster buccal pouch, in order to reduce the period of latency for tumor formation. METHODS: Sixteen hamsters were randomized into two groups of eight animals each. The hamsters of the group I had their right buccal pouches treated with 0.5% DMBA and 10% carbamide peroxide teeth bleaching gel for 55 days. The animals of the group II had their right pouches treated only with DMBA. After, six animals of each group had their pouches prepared for light microscopy. Histomorphometry was performed to assess the presence of keratinization, nuclear polymorphism, pattern of invasion, number of blood vessels, and inflammatory infiltrate in the tumor front. Furthermore, the newly formed lesions were graded according the Bryne's grading system. The remaining animals had the vascular system of the pouches casted by Mercox and qualitatively analyzed by scanning electron microscopy. RESULTS: CONCLUSION: The protocol presented here, using DMBA associated with carbamide peroxide, shortens the period of latency to produce squamous cell carcinoma in the hamster buccal pouch, decreasing the time and costs of the experiments.
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Authors | Vinícius Faccin Bampi, Wadson Ferreira Vilela, Reggiani Vilela Gonçalves, Maria Gabriela Tavares Rheingantz, Luiz Fernando Minello, Jefferson Luis Braga da Silva, Laura Beatriz Oliveira de Oliveira |
Journal | Clinical and experimental otorhinolaryngology
(Clin Exp Otorhinolaryngol)
Vol. 7
Issue 3
Pg. 210-5
(Sep 2014)
ISSN: 1976-8710 [Print] Korea (South) |
PMID | 25177438
(Publication Type: Journal Article)
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