Abstract | INTRODUCTION: Platelet-rich plasma (PRP) has been described as platelet concentrate. Growth factors released by activated platelets can improve wound vasculogenesis and enhance wound healing. In this study, we used PRP instead of serum to culture human dental pulp stem cells (hDPSCs) and endothelial progenitor cells (EPCs) and investigated revascularization ability. The effect of hDPSC and EPC coculture on vasculogenesis was also studied. METHODS: RESULTS: On day 3, the expression levels of all the markers in the coculture groups were much higher than in the single-culture groups and were also higher in the PRP groups compared with the FBS groups (P < .05), except for SDF-1. Expression levels were significantly higher in the experimental groups (EPCs + 10% PRP, EPCs + hDPSCs + 10% FBS, and EPCs + hDPSCs + 10% PRP) than in the control group (EPCs + 10% FBS) and in the PRP groups/coculture groups compared with the FBS groups/single-culture groups (P < .01). The tube formation assay showed the area of vessel-like structures formed by the PRP group to be larger than in the FBS group (P < .05). CONCLUSIONS: PRP and coculture can both promote vasculogenesis, and PRP can promote EPCs to form vessel-like structures.
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Authors | Xinzhu Li, Jin Hou, Buling Wu, Ting Chen, Aoxiang Luo |
Journal | Journal of endodontics
(J Endod)
Vol. 40
Issue 11
Pg. 1810-4
(Nov 2014)
ISSN: 1878-3554 [Electronic] United States |
PMID | 25175848
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Angiogenic Proteins
- Chemokine CXCL12
- Platelet-Derived Growth Factor
- VEGFA protein, human
- Vascular Endothelial Growth Factor A
- Vascular Endothelial Growth Factor Receptor-2
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Topics |
- Adolescent
- Adult
- Angiogenic Proteins
(analysis)
- Cell Culture Techniques
- Cells, Cultured
- Chemokine CXCL12
(analysis)
- Child
- Coculture Techniques
- Dental Pulp
(cytology)
- Endothelial Cells
(physiology)
- Fetal Blood
- Humans
- Microvessels
(growth & development)
- Neovascularization, Physiologic
(physiology)
- Platelet-Derived Growth Factor
(analysis)
- Platelet-Rich Plasma
(physiology)
- Stem Cells
(physiology)
- Vascular Endothelial Growth Factor A
(analysis)
- Vascular Endothelial Growth Factor Receptor-2
(analysis)
- Young Adult
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