Antioxidant activity and protective effect of extract of Celosia cristata L. flower on tert-butyl hydroperoxide-induced oxidative hepatotoxicity.

Abstract

This study was undertaken to evaluate the antioxidant potential and protective effects of Celosia cristata L. (Family: Amaranthaceae) flower (CCF) extracts on tert-butyl-hydroperoxide (t-BHP)-induced oxidative damage in the hepatocytes of Chang cells and rat livers. In vitro, CCF extracts exhibited protective effect through their radical scavenging ability to enhance cell viability, prevent reactive oxygen species (ROS) generation, and inhibit mitochondrial membrane depolarisation in t-BHP-induced hepatotoxicity in Chang cells. In vivo, oral feeding of CCF (100mg and 500mg/kg of body weight) to rats for five consecutive days before a single dose of t-BHP (2mmol/kg, i.p.) showed a significant (p<0.05) protective effect by lowering serum levels of glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT). The extract decreased the hepatic levels of lipid peroxidation (MDA) and serum level of triglyceride (TG) against t-BHP-induced oxidative stress. These results indicate that CCF extract prevented oxidative stress-induced liver injury by enhancing hepatocyte antioxidant abilities.

• Authors: Yon-Suk Kim, Jin-Woo Hwang, Si-Heung Sung, You-Jin Jeon, Jae-Hyun Jeong, Byong-Tae Jeon, Sang-Ho Moon, Pyo-Jam Park
• Journal: Food chemistry (Food Chem) Vol. 168 Pg. 572-9 (Feb 01 2015) ISSN: 1873-7072 [Electronic] England
• PMID: 25172750 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014. Published by Elsevier Ltd.

Chemical References

• Antioxidants
• Plant Extracts
• Protective Agents
• Reactive Oxygen Species
• tert-Butylhydroperoxide

Topics

• Animals
• Antioxidants (chemistry, pharmacology)
• Cell Line
• Cell Survival (drug effects)
• Celosia (chemistry, metabolism)
• Chemical and Drug Induced Liver Injury (metabolism, pathology, prevention & control)
• Flowers (chemistry, metabolism)
• Hepatocytes (cytology, drug effects)
• Lipid Peroxidation (drug effects)
• Male
• Membrane Potential, Mitochondrial (drug effects)
• Oxidative Stress (drug effects)
• Plant Extracts (chemistry, pharmacology)
• Protective Agents (chemistry, pharmacology)
• Rats
• Rats, Sprague-Dawley
• Reactive Oxygen Species (metabolism)
• tert-Butylhydroperoxide (toxicity)