Abstract | BACKGROUND: Dominant mutations in superoxide dismutase 1 (SOD1) cause degeneration of motor neurons in a subset of inherited amyotrophic lateral sclerosis (ALS). The pathogenetic process mediated by misfolded and/or aggregated mutant SOD1 polypeptides is hypothesized to be suppressed by protein refolding. This genetic study is aimed to test whether mutant SOD1-mediated ALS pathology recapitulated in mice could be alleviated by overexpressing a longevity-related deacetylase SIRT1 whose substrates include a transcription factor heat shock factor 1 (HSF1), the master regulator of the chaperone system. RESULTS: We established a line of transgenic mice that chronically overexpress SIRT1 in the brain and spinal cord. While inducible HSP70 (HSP70i) was upregulated in the spinal cord of SIRT1 transgenic mice (PrP-Sirt1), no neurological and behavioral alterations were detected. To test hypothetical benefits of SIRT1 overexpression, we crossbred PrP-Sirt1 mice with two lines of ALS model mice: A high expression line that exhibits a severe phenotype (SOD1G93A-H) or a low expression line with a milder phenotype (SOD1G93A-L). The Sirt1 transgene conferred longer lifespan without altering the time of symptomatic onset in SOD1G93A-L. Biochemical analysis of the spinal cord revealed that SIRT1 induced HSP70i expression through deacetylation of HSF1 and that SOD1G93A-L/PrP- Sirt1 double transgenic mice contained less insoluble SOD1 than SOD1G93A-L mice. Parallel experiments showed that Sirt1 transgene could not rescue a more severe phenotype of SOD1G93A-H transgenic mice partly because their HSP70i level had peaked out. CONCLUSIONS: The genetic supplementation of SIRT1 can ameliorate a mutant SOD1-linked ALS mouse model partly through the activation of the HSF1/HSP70i chaperone system. Future studies shall include testing potential benefits of pharmacological enhancement of the deacetylation activity of SIRT1 after the onset of the symptom.
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Authors | Seiji Watanabe, Natsumi Ageta-Ishihara, Shinji Nagatsu, Keizo Takao, Okiru Komine, Fumito Endo, Tsuyoshi Miyakawa, Hidemi Misawa, Ryosuke Takahashi, Makoto Kinoshita, Koji Yamanaka |
Journal | Molecular brain
(Mol Brain)
Vol. 7
Pg. 62
(Aug 29 2014)
ISSN: 1756-6606 [Electronic] England |
PMID | 25167838
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA-Binding Proteins
- HSF1 protein, human
- HSP70 Heat-Shock Proteins
- Heat Shock Transcription Factors
- SOD1 protein, human
- Transcription Factors
- Sod1 protein, mouse
- Superoxide Dismutase
- Superoxide Dismutase-1
- Sirt1 protein, mouse
- Sirtuin 1
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Topics |
- Acetylation
- Amyotrophic Lateral Sclerosis
(metabolism, pathology)
- Animals
- Behavior, Animal
- DNA-Binding Proteins
(metabolism)
- Disease Models, Animal
- Gene Dosage
- HSP70 Heat-Shock Proteins
(metabolism)
- Heat Shock Transcription Factors
- Humans
- Longevity
- Mice, Inbred C57BL
- Mice, Transgenic
- Mutation
(genetics)
- Promoter Regions, Genetic
(genetics)
- Protein Folding
- Sirtuin 1
(metabolism)
- Spinal Cord
(pathology)
- Superoxide Dismutase
(genetics)
- Superoxide Dismutase-1
- Transcription Factors
(metabolism)
- Up-Regulation
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