The aim of the present research is to evaluate the antiurolithiatic effect of the various extracts prepared from the fruits of Viburnum opulus L., in regard to its ethnobotanical record. To induce
urolithiasis, 70 mg/kg
sodium oxalate was injected to the rats which were housed individually in metabolic cages. The test materials were applied during 7 days. Biochemical (urine and serum parameters), histopathological and
antioxidant (
TBARs, TSH and GSH) assays were conducted. The urine samples were examined by light microscope for the determination of the
calcium oxalate crystals. Lyophilized juice of V. opulus (LJVO) and lyophilized commercial juice of V. opulus (LCJVO) exerted potential antiurolithiatic activity which was attributed to its
diuretic effect along with the inhibitory action on the
oxalate levels and
free radical production. We also determined the
chlorogenic acid content of the LJVO by high-performance liquid chromatography (HPLC).
Chlorogenic acid was determined by using Supelcosil LC-18 (250 × 4.6 mm, 5 µm) column and
acetonitrile: water: 0.2% o-
phosphoric acid as a mobile phase. The
chlorogenic acid content of V. opulus was found to be 0.3227 mg/mL in fruit juice. The results obtained in this study have provided a scientific evidence for the traditional usage of V. opulus on passing
kidney stones in Turkish
folk medicine.