HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

The pyruvate-tricarboxylic acid cycle node: a focal point of virulence control in the enteric pathogen Yersinia pseudotuberculosis.

Abstract
Despite our increasing knowledge of the specific pathogenicity factors in bacteria, the contribution of metabolic processes to virulence is largely unknown. Here, we elucidate a tight connection between pathogenicity and core metabolism in the enteric pathogen Yersinia pseudotuberculosis by integrated transcriptome and [(13)C]fluxome analysis of the wild type and virulence-regulator mutants. During aerobic growth on glucose, Y. pseudotuberculosis reveals an unusual flux distribution with a high level of secreted pyruvate. The absence of the transcriptional and post-transcriptional regulators RovA, CsrA, and Crp strongly perturbs the fluxes of carbon core metabolism at the level of pyruvate metabolism and the tricarboxylic acid (TCA) cycle, and these perturbations are accompanied by transcriptional changes in the corresponding enzymes. Knock-outs of regulators of this metabolic branch point and of its central enzyme, pyruvate kinase (ΔpykF), result in mutants with significantly reduced virulence in an oral mouse infection model. In summary, our work identifies the pyruvate-TCA cycle node as a focal point for controlling the host colonization and virulence of Yersinia.
AuthorsRené Bücker, Ann Kathrin Heroven, Judith Becker, Petra Dersch, Christoph Wittmann
JournalThe Journal of biological chemistry (J Biol Chem) Vol. 289 Issue 43 Pg. 30114-32 (Oct 24 2014) ISSN: 1083-351X [Electronic] United States
PMID25164818 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.
Chemical References
  • Bacterial Proteins
  • Pyruvates
  • Iron
  • Glucose
Topics
  • Adaptation, Physiological (drug effects, genetics)
  • Animals
  • Bacterial Proteins (genetics, metabolism)
  • Biomass
  • Citric Acid Cycle (drug effects, genetics)
  • Escherichia coli (drug effects, metabolism)
  • Female
  • Gene Expression Regulation, Bacterial (drug effects)
  • Gene Regulatory Networks
  • Glucose (pharmacology)
  • Iron (pharmacology)
  • Metabolic Flux Analysis
  • Mice
  • Mice, Inbred BALB C
  • Molecular Weight
  • Mutation (genetics)
  • Pyruvates (metabolism)
  • Stress, Physiological (drug effects, genetics)
  • Transcriptome (drug effects, genetics)
  • Virulence (drug effects, genetics)
  • Yersinia pseudotuberculosis (genetics, growth & development, metabolism, pathogenicity)
  • Yersinia pseudotuberculosis Infections (microbiology, pathology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: