Synthesis and evaluation of glycopolymeric decorated gold nanoparticles functionalized with gold-triphenyl phosphine as anti-cancer agents.

Abstract

In this study, statistical glyco-dithiocarbamate (DTC) copolymers were synthesized by reversible addition-fragmentation chain transfer polymerization (RAFT) and subsequently used to prepare glyconanoparticles and conjugated glyconanoparticles with the anticancer drug, gold(I) triphenylphosphine. These glyconanoparticles and the corresponding conjugates were then tested for their in vitro cytotoxicity in both normal and cancer cell lines using Neutral Red assay. The glyconanoparticles and their Au(I)PPh3 conjugates were all active against MCF7 and HepG2 cells, but galactose-functionalized glyconanoparticles {P(GMA-EDAdtc(AuPPh3)-st-LAEMA)AuNP} were found to be the most cytotoxic to HepG2 cells (IC50 ∼ 4.13 ± 0.73 μg/mL). The p(GMA-EDAdtc(AuPPh3)-st-LAEMA)AuNP was found to be a 4-fold more potent antitumor agent in HepG2 cells, and the overexpressed asialoglycoprotein (ASGPR) receptors revealed to play an important role in the cytotoxicity, presumably by the enhanced uptake. In addition, the glyconanoparticles Au(I) conjugates are found to be significantly more toxic as compared to the standard chemotherapeutic reagents such as cisplatin and cytarabine.

• Authors: Christian K Adokoh, Stephen Quan, Mary Hitt, James Darkwa, Piyush Kumar, Ravin Narain
• Journal: Biomacromolecules (Biomacromolecules) Vol. 15 Issue 10 Pg. 3802-10 (Oct 13 2014) ISSN: 1526-4602 [Electronic] United States
• PMID: 25162942 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Organophosphorus Compounds
• Polymers
• Cytarabine
• triphenylphosphine
• Gold
• Cisplatin

Topics

• Antineoplastic Agents (chemistry, pharmacology)
• Cell Line, Tumor
• Cisplatin (chemistry, pharmacology)
• Cytarabine (chemistry, pharmacology)
• Drug Screening Assays, Antitumor (methods)
• Gold (chemistry, pharmacology)
• HeLa Cells
• Hep G2 Cells
• Humans
• MCF-7 Cells
• Metal Nanoparticles (administration & dosage, chemistry)
• Organophosphorus Compounds (chemistry, pharmacology)
• Polymerization
• Polymers (chemistry, pharmacology)