Abstract |
Diets containing deoxynivalenol (DON) were fed to rainbow trout Oncorhynchus mykiss (Walbaum) for 4 weeks followed by experimental infection (intraperitoneal) with Flavobacterium psychrophilum (4.1 × 10(6) colony-forming units [CFU] mL(-1) ). Mortality of rainbow trout fed either 6.4 mg kg(-1) DON or trout pair-fed the control diet was significantly reduced (P < 0.05) in comparison with trout fed the control diet to apparent satiation (<0.1 mg kg(-1) DON). In a second experiment, trout were fed one of three experimental diets; a control diet, a diet produced with corn naturally contaminated with DON (3.3 mg kg(-1) DON) or a diet containing purified DON (3.8 mg kg(-1) ); however, these fish were not experimentally infected. The presence of DON resulted in significant reduction (P < 0.0001) in feed intake as well as weight gain after 4 weeks. Respiratory burst of head-kidney leucocytes isolated from rainbow trout fed diets containing purified DON (3.8 mg kg(-1) ) was significantly higher (P < 0.05) at 35 day post-exposure compared with controls. The antimicrobial activity of DON was examined by subjecting F. psychrophilum in vitro to serial dilutions of the chemical. Complete inhibition occurred at a concentration of 75 mg L(-1) DON, but no effect was observed below this concentration (0-30 mg L(-1) ).
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Authors | I A Ryerse, J M Hooft, D P Bureau, M A Hayes, J S Lumsden |
Journal | Journal of fish diseases
(J Fish Dis)
Vol. 38
Issue 9
Pg. 809-19
(Sep 2015)
ISSN: 1365-2761 [Electronic] England |
PMID | 25160820
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 John Wiley & Sons Ltd. |
Chemical References |
- Toxins, Biological
- Trichothecenes
- deoxynivalenol
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Topics |
- Animals
- Caloric Restriction
- Fish Diseases
(drug therapy, microbiology, mortality)
- Flavobacteriaceae Infections
(drug therapy, microbiology, mortality, veterinary)
- Flavobacterium
(drug effects, growth & development)
- Oncorhynchus mykiss
(microbiology)
- Toxins, Biological
(pharmacology, therapeutic use)
- Trichothecenes
(isolation & purification, pharmacology)
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